Anti-SCD1 antibody (ab39969)
Key features and details
- Rabbit polyclonal to SCD1
- Suitable for: ICC, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-SCD1 antibody
See all SCD1 primary antibodies -
Description
Rabbit polyclonal to SCD1 -
Host species
Rabbit -
Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab236868.
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Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Pig -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HepG2 and HEK293 cell lysates. ICC: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 5 µg/ml.
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WB | (1) |
Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 41.5 kDa).
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Notes |
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ICC
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 41.5 kDa). |
Target
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Function
Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. -
Sequence similarities
Belongs to the fatty acid desaturase family. -
Domain
The histidine box domains may contain the active site and/or be involved in metal ion binding. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 6319 Human
- Entrez Gene: 20249 Mouse
- Omim: 604031 Human
- SwissProt: O00767 Human
- SwissProt: P13516 Mouse
- Unigene: 558396 Human
- Unigene: 597496 Human
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Alternative names
- ACOD_HUMAN antibody
- Acyl-CoA desaturase antibody
- Delta(9)-desaturase antibody
see all
Images
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All lanes : Anti-SCD1 antibody (ab39969) at 1 µg/ml
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 3 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4 : Empty Lane
Lane 5 : SCD knockout HeLa whole cell lysate at 20 µg
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 41.5 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab39969 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to IRDye® 800CW (green).
Loading control: alpha Tubulin ab7291 at 0.1 µg/mL visualised using a Goat anti-Mouse IgG H&L (IRDye® 680RD, red).
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All lanes : Anti-SCD1 antibody (ab39969) at 2 µg/ml
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SCD knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 41.5 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab39969 observed at 36 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab39969 was shown to react with SCD1 in wild-type HeLa cells in western blot with loss of signal observed in SCD1 knockout cell line ab265220 (SCD1 knockout cell lysate ab257658). Wild-type and SCD1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab39969 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at 2 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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ab39969 staining SCD1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab39969 at 5 µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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All lanes : Anti-SCD1 antibody (ab39969) at 2 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 41.5 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab39969 has been referenced in 18 publications.
- Zhang Q et al. Bailing capsule (Cordyceps sinensis) ameliorates renal triglyceride accumulation through the PPARα pathway in diabetic rats. Front Pharmacol 13:915592 (2022). PubMed: 36091833
- Gao WY et al. Tanshinone IIA Downregulates Lipogenic Gene Expression and Attenuates Lipid Accumulation through the Modulation of LXRa/SREBP1 Pathway in HepG2 Cells. Biomedicines 9:N/A (2021). PubMed: 33806955
- Liu L & Lan S Interference of S100A16 suppresses lipid accumulation and inflammation in high glucose-induced HK-2 cells. Int Urol Nephrol 53:1255-1263 (2021). PubMed: 33389513
- Thomas T et al. Fatty acid desaturase activity in mature red blood cells and implications for blood storage quality. Transfusion 61:1867-1883 (2021). PubMed: 33904180
- Abd Alla J et al. Stearoyl-CoA Desaturase (SCD) Induces Cardiac Dysfunction with Cardiac Lipid Overload and Angiotensin II AT1 Receptor Protein Up-Regulation. Int J Mol Sci 22:N/A (2021). PubMed: 34576047