Overview

  • Product nameAnti-SCD1 antibody [CD.E10]
    See all SCD1 primary antibodies
  • Description
    Mouse monoclonal [CD.E10] to SCD1
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, ICC/IF, Flow Cyt, WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • NIH 3T3 cells
  • General notesSCD1 is known to undergo post-translational modifications and the sizes differ in different cell lines so the observed band size can be different than predicted band size. As positive control we recommend using SCD1 over-expressed 293 transfected cell lysates for western blot.

Properties

Applications

Our Abpromise guarantee covers the use of ab19862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 4 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 20530718
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB 1/1000. Predicted molecular weight: 41.5 kDa. PubMed: 18697866
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • FunctionTerminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA.
  • Sequence similaritiesBelongs to the fatty acid desaturase family.
  • DomainThe histidine box domains may contain the active site and/or be involved in metal ion binding.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACOD_HUMAN antibody
    • Acyl-CoA desaturase antibody
    • Delta(9)-desaturase antibody
    • Delta-9 desaturase antibody
    • Delta-9-Desaturase antibody
    • FADS5 antibody
    • Fatty acid desaturase antibody
    • PRO0998 antibody
    • SCD 1 antibody
    • SCD antibody
    • SCD1 antibody
    • Stearoyl-CoA desaturase antibody
    see all

Anti-SCD1 antibody [CD.E10] images



  • Predicted band size : 41.5 kDa


    Western blot using ab19862 on 293 cells.
  • SCD was immunoprecipitated using 0.5mg Hek293 whole cell extract, 10µg of Mouse monoclonal to SCD and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab19862.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 32kDa; SCD.
  • ab19862 staining SCD in human skin.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ICC/IF image of ab19862 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19862, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HepG2 cells stained with ab19862 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19862, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-SCD1 antibody [CD.E10] (ab19862)

This product has been referenced in:
  • Lu Y  et al. Overexpression of stearoyl-CoA desaturase 1 in bone marrow mesenchymal stem cells enhance the expression of induced endothelial cells. Lipids Health Dis 13:53 (2014). WB . Read more (PubMed: 24650127) »
  • Wang C  et al. Long-term supranutritional supplementation with selenate decreases hyperglycemia and promotes fatty liver degeneration by inducing hyperinsulinemia in diabetic db/db mice. PLoS One 9:e101315 (2014). WB . Read more (PubMed: 24983750) »

See all 16 Publications for this product

Product Wall

Application Flow Cytometry
Fixation Formaldehyde
Permeabilization Yes - 0.1% PBS-Tween
Sample Rat Cell (liver)
Specification liver
Gating Strategy CD24 CELLS
Preparation Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: 0.1% PBS BSA
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Submitted Dec 10 2013

Application Flow Cytometry
Fixation Formaldehyde
Permeabilization Yes - BD PERMEABILISATION BUFFER
Sample Human Cell (HEP G2)
Specification HEP G2
Gating Strategy CD24 CELLS
Preparation Cell harvesting/tissue preparation method: single cell suspension
Sample buffer: 0.1% PBS BSA
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Submitted Dec 06 2013

Application Western blot
Loading amount 5 µg
Gel Running Conditions Reduced Denaturing (8)
Sample Human Cell lysate - whole cell (LIVER)
Specification LIVER
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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Submitted Dec 05 2013

Gracias por contactarnos.

Espero que no te importe que te conteste en castellano. Recientemente hemos establecido la Oficina Virtual Española y podéis contactarnos en este idioma siempre que lo deseéis. Si preferís...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In or...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Rat kidney tissue)
Loading amount 140 µg
Specification Rat kidney tissue
Gel Running Conditions Reduced Denaturing (12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 30 2012

Thank you for contacting us. I am sorry that this antibody is detecting multiple bands in WB on rat samples.
Is the secondary antibody you are using pre-adsorbed against rat? It is possible that the anti-mouse secondary may be detecting the heavy ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for calling Abcam. I have continued the search for the guinea pig sequence for stearyl-CoA desaturase, but I have not been able to locate it. I can find, rat, mouse, sheep, goat, bovine, hamster but not guinea pig. Therefore I cannot do an al...

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Thank you for your email. We highly appreciate your cooperation in this. I am sorry we are unable to resolve the problems you have been experiencing. It may be possible that these antibodies are not suitable for Typhoon method with Kpi lysis buf...

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