Overview

  • Product name
    Anti-SCE1 antibody
  • Description
    Rabbit polyclonal to SCE1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Arabidopsis thaliana
  • Immunogen

    Full length protein produced in E.coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab98965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 18 kDa.

Target

  • Relevance
    SUMO-conjugating enzyme accepts the SUMO proteins from the E1 SUMO-activating heterodimer SAE1/SAE2 and catalyzes its covalent attachment to other proteins with the E3 SUMO ligases SIZ1 and MMS21. It associates with SIZ1 for sumoylation of the transcription factor GTE3.
  • Database links
    • Alternative names
      • AHUS5 antibody
      • AT3G57870 antibody
      • ATSCE1 antibody
      • EMB1637 antibody
      • EMBRYO DEFECTIVE 1637 antibody
      • SCE1A antibody
      • SUMO CONJUGATING ENZYME 1A antibody
      • SUMO CONJUGATION ENZYME 1 antibody
      • SUMO ligase antibody
      see all

    Images

    • All lanes : Anti-SCE1 antibody (ab98965) at 1/1000 dilution

      Lane 1 : Whole leaf tissue lysate prepared from wild type Arabidopsis thaliana Col-0
      Lane 2 : Whole leaf tissue lysate prepared from Arabidopsis thaliana overexpressing SCE1

      Secondary
      HRP conjugated goat anti-rabbit polyclonal at 1/5000 dilution
      Developed using the ECL technique

      Predicted band size : 18 kDa
      Observed band size : 18 kDa
      Additional bands at : 28 kDa,32 kDa,50 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 1 minute

      Image courtesy of Michael Skelly by Abreview.

      See Abreview

    References

    This product has been referenced in:
    • Xiong R & Wang A SCE1, the SUMO-conjugating enzyme in plants that interacts with NIb, the RNA-dependent RNA polymerase of Turnip mosaic virus, is required for viral infection. J Virol 87:4704-15 (2013). Read more (PubMed: 23365455) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Arabidopsis thaliana Tissue lysate - whole (Arabidopsis leaf tissue)
    Loading amount
    200000 µg
    Specification
    Arabidopsis leaf tissue
    Gel Running Conditions
    Non-reduced Denaturing (15% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Mr. Michael Skelly

    Verified customer

    Submitted Aug 24 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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