1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
May function in the assembly or regulation of proteins in the cornified envelope. The LIM domain may be involved in homotypic or heterotypic associations and may function to localize sciellin to the cornified envelope.
Highly expressed in esophagus. It is also expressed in keratinocytes, amniotic tissue, foreskin stratum spinosum and stratum granulosum, hair follicle and nail.
Contains 1 LIM zinc-binding domain.
Cytoplasm. Membrane. May become cross-linked to membrane proteins by transglutaminase.
Flow cytometric analysis of A549 cells labeling SCEL with ab170476 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot - Anti-SCEL antibody (ab170476)
All lanes : Anti-SCEL antibody (ab170476) at 1/100 dilution
Lane 1 : NCI-H292 cell lysate Lane 2 : A549 cell lysate