Overview

  • Product name
  • Description
    Rabbit polyclonal to SCEL
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human SCEL aa 247-277 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q95171

  • Positive control
    • NCI-H292 and A549 cell lysates; A549 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab170476 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 78 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    May function in the assembly or regulation of proteins in the cornified envelope. The LIM domain may be involved in homotypic or heterotypic associations and may function to localize sciellin to the cornified envelope.
  • Tissue specificity
    Highly expressed in esophagus. It is also expressed in keratinocytes, amniotic tissue, foreskin stratum spinosum and stratum granulosum, hair follicle and nail.
  • Sequence similarities
    Contains 1 LIM zinc-binding domain.
  • Cellular localization
    Cytoplasm. Membrane. May become cross-linked to membrane proteins by transglutaminase.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ21667 antibody
    • MGC22531 antibody
    • Scel antibody
    • SCEL_HUMAN antibody
    • Sciellin antibody
    see all

Images

  • Flow cytometric analysis of A549 cells labeling SCEL with ab170476 at 1/10 dilution (right histogram)  compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • All lanes : Anti-SCEL antibody (ab170476) at 1/100 dilution

    Lane 1 : NCI-H292 cell lysate
    Lane 2 : A549 cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 78 kDa

References

ab170476 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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