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Highly pure (>98%) recombinant Rat SCF.
Our Abpromise guarantee covers the use of ab101072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Neutralising||Use a concentration of 0.08 - 0.12 µg/ml. Required to yield one half maximal inhibition [ND50] of the biological activity of Rat SCF (15 ng/ml)|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 31 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat SCF is 1.5-3.0 ng/lane, under either reducing or non reducing conditions.|
|Indirect ELISA||Use a concentration of 0.5 - 2 µg/ml. Using 100 µl/well antibody solution, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat SCF in conjunction with compatible secondary reagents.|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Using 100 µl/well antibody solution, in conjunction with Biotinylated Anti-Rat SCF as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat SCF.|
ab101072 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"