Overview

  • Product nameAnti-SCF antibody
    See all SCF primary antibodies
  • Description
    Rabbit polyclonal to SCF
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, African Green Monkey
    Predicted to work with: Rat, Sheep, Goat, Horse, Cow, Cat, Dog, Pig, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human SCF.

    (Peptide available as ab73180.)

  • Positive control
    • This antibody gave a positive signal in the following Lysates: EBS E14 TG2A Day 3 Whole Cell, Raji Whole Cell, SK N SH Whole Cell, Human Brain Tissue, HEK293 Whole Cell, A498 Whole Cell

Properties

Applications

Our Abpromise guarantee covers the use of ab64677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 31 kDa).

Target

  • FunctionLigand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
  • Involvement in diseaseHyperpigmentation with or without hypopigmentation, familial progressive
    Deafness, congenital, unilateral or asymmetric
  • Sequence similaritiesBelongs to the SCF family.
  • Developmental stageActs in the early stages of hematopoiesis.
  • Post-translational
    modifications
    A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
    Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
    A soluble form exists as a cleavage product of the extracellular domain.
  • Cellular localizationSecreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
  • Information by UniProt
  • Database links
  • Alternative names
    • C kit ligand antibody
    • C-kit ligand antibody
    • Ckit ligand antibody
    • DCUA antibody
    • DFNA69 antibody
    • DKFZp686F2250 antibody
    • familial progressive hyperpigmentation 2 antibody
    • FPH2 antibody
    • FPHH antibody
    • KIT ligand antibody
    • Kitl antibody
    • KITLG antibody
    • KL 1 antibody
    • KL1 antibody
    • Mast cell growth factor antibody
    • MGF antibody
    • MGF stem cell factor antibody
    • SCF antibody
    • SCF_HUMAN antibody
    • SF antibody
    • SHEP7 antibody
    • sKITLG antibody
    • Soluble KIT ligand antibody
    • Steel factor antibody
    • steel, mouse, homolog of antibody
    • Stem cell factor antibody
    • Stem cell factor precursor antibody
    see all

Anti-SCF antibody images

  • All lanes : Anti-SCF antibody (ab64677) at 1 µg/ml

    Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
    Lane 4 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080)

    Predicted band size : 31 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • IHC image of SCF staining in human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64677, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ICC/IF image of ab64677 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64677, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 10µg/ml.

References for Anti-SCF antibody (ab64677)

This product has been referenced in:
  • Nishikori Y  et al. The role of mast cells in cutaneous wound healing in streptozotocin-induced diabetic mice. Arch Dermatol Res 306:823-35 (2014). Mouse . Read more (PubMed: 25218083) »
  • Kim EJ  et al. Platycarya strobilacea S. et Z. Extract Has a High Antioxidant Capacity and Exhibits Hair Growth-promoting Effects in Male C57BL/6 Mice. Prev Nutr Food Sci 19:136-44 (2014). IHC ; Mouse . Read more (PubMed: 25320710) »

See all 5 Publications for this product

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Expiration date: June 11, 2012

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample African Green Monkey Cell (COS cell line)
Specification COS cell line
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 02 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse Ovary)
Specification Mouse Ovary
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris Buffer pH 10.0
Permeabilization Yes - Tween20
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
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Submitted May 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (293FT cell line)
Specification 293FT cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Mar 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"