Overview

  • Product nameAnti-SCF antibody
    See all SCF primary antibodies
  • Description
    Rabbit polyclonal to SCF
  • Tested applicationsSuitable for: WB, ELISA, Neutralisingmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Highly pure (>98%) recombinant mSCF (mouse Stem Cell Factor)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab9753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml.
Neutralising Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of mSCF (25.0 ng/ml), a concentration of 0.6 - 0.65 µg/ml of this antibody is required.

Target

  • FunctionLigand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
  • Involvement in diseaseHyperpigmentation with or without hypopigmentation, familial progressive
    Deafness, congenital, unilateral or asymmetric
  • Sequence similaritiesBelongs to the SCF family.
  • Developmental stageActs in the early stages of hematopoiesis.
  • Post-translational
    modifications
    A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
    Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
    A soluble form exists as a cleavage product of the extracellular domain.
  • Cellular localizationSecreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
  • Information by UniProt
  • Database links
  • Alternative names
    • C kit ligand antibody
    • C-kit ligand antibody
    • Ckit ligand antibody
    • DCUA antibody
    • DFNA69 antibody
    • DKFZp686F2250 antibody
    • familial progressive hyperpigmentation 2 antibody
    • FPH2 antibody
    • FPHH antibody
    • KIT ligand antibody
    • Kitl antibody
    • KITLG antibody
    • KL 1 antibody
    • KL1 antibody
    • Mast cell growth factor antibody
    • MGF antibody
    • MGF stem cell factor antibody
    • SCF antibody
    • SCF_HUMAN antibody
    • SF antibody
    • SHEP7 antibody
    • sKITLG antibody
    • Soluble KIT ligand antibody
    • Steel factor antibody
    • steel, mouse, homolog of antibody
    • Stem cell factor antibody
    • Stem cell factor precursor antibody
    see all

References for Anti-SCF antibody (ab9753)

This product has been referenced in:
  • Duran JM  et al. Bone-derived stem cells repair the heart after myocardial infarction through transdifferentiation and paracrine signaling mechanisms. Circ Res 113:539-52 (2013). WB ; Mouse . Read more (PubMed: 23801066) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"