Overview

  • Product name
    Anti-SCF antibody (Biotin)
    See all SCF primary antibodies
  • Description
    Goat polyclonal to SCF (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: ELISA, Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant full length Human protein

Properties

Applications

Our Abpromise guarantee covers the use of ab84444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Sandwich ELISA
WB
  • Application notes
    ELISA: Use at a concentration of 0.25 - 1 µg/ml. Detection limit for recombinant Human SCF: 0.2 0.4 ng/well, using 100 µl/well antibody solution.
    sELISA: Use at a concentration of 0.25 - 1 µg/ml. Detection limit for recombinant Human SCF: 0.2 0.4 ng/well, using 100 µl/well antibody solution.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Detection limit for recombinant Human SCF: 1.5 - 3.0 ng/lane, under reducing or non-reducing conditions. Predicted molecular weight: 31 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
    • Involvement in disease
      Hyperpigmentation with or without hypopigmentation, familial progressive
      Deafness, congenital, unilateral or asymmetric
    • Sequence similarities
      Belongs to the SCF family.
    • Developmental stage
      Acts in the early stages of hematopoiesis.
    • Post-translational
      modifications
      A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
      Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
      A soluble form exists as a cleavage product of the extracellular domain.
    • Cellular localization
      Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
    • Information by UniProt
    • Database links
    • Alternative names
      • C kit ligand antibody
      • C-kit ligand antibody
      • Ckit ligand antibody
      • DCUA antibody
      • DFNA69 antibody
      • DKFZp686F2250 antibody
      • familial progressive hyperpigmentation 2 antibody
      • FPH2 antibody
      • FPHH antibody
      • KIT ligand antibody
      • Kitl antibody
      • KITLG antibody
      • KL 1 antibody
      • KL1 antibody
      • Mast cell growth factor antibody
      • MGF antibody
      • MGF stem cell factor antibody
      • SCF antibody
      • SCF_HUMAN antibody
      • SF antibody
      • SHEP7 antibody
      • sKITLG antibody
      • Soluble KIT ligand antibody
      • Steel factor antibody
      • steel, mouse, homolog of antibody
      • Stem cell factor antibody
      • Stem cell factor precursor antibody
      see all

    References

    ab84444 has not yet been referenced specifically in any publications.

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