Overview

  • Product name
  • Description
    Rabbit polyclonal to SCFD1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within internal sequence amino acids 129-365 of Human SCFD1.

  • Positive control
    • IMR32, U87-MG and MCF-7 cell lysates; J5 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function
    Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. Involved in vesicular transport between the endoplasmic reticulum and the Golgi.
  • Sequence similarities
    Belongs to the STXBP/unc-18/SEC1 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C14orf163 antibody
    • Chromosome 14 open reading frame 163 antibody
    • RA410 antibody
    • Scfd1 antibody
    • SCFD1_HUMAN antibody
    • Sec1 family domain containing 1 antibody
    • Sec1 family domain-containing protein 1 antibody
    • SLY1 antibody
    • SLY1 homolog antibody
    • Sly1p antibody
    • STXBP1L2 antibody
    • Syntaxin binding protein 1 like 2 antibody
    • Syntaxin-binding protein 1-like 2 antibody
    • Vesicle transport related protein antibody
    see all

Images

  • Anti-SCFD1 antibody (ab154096) at 1/5000 dilution + U87-MG whole cell lysate at 30 µg

    Predicted band size: 72 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded J5 xenograft tissue labeling SCFD1 with ab154096 at 1/500 dilution.

References

ab154096 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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