Overview

  • Product nameAnti-SCFD1 antibody
    See all SCFD1 primary antibodies
  • Description
    Rabbit polyclonal to SCFD1
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 300 - 350 (VENSPAGARP KRKNKKSYDL TPVDKFWQKH KGSPFPEVAE SVQQELESYR A) of Human SCFD1 (NP_057190.2)

  • Positive control
    • Whole cell lysate from HeLa cells, 293T cells or NIH3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab86594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 72 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionPlays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. Involved in vesicular transport between the endoplasmic reticulum and the Golgi.
  • Sequence similaritiesBelongs to the STXBP/unc-18/SEC1 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C14orf163 antibody
    • Chromosome 14 open reading frame 163 antibody
    • RA410 antibody
    • Scfd1 antibody
    • SCFD1_HUMAN antibody
    • Sec1 family domain containing 1 antibody
    • Sec1 family domain-containing protein 1 antibody
    • SLY1 antibody
    • SLY1 homolog antibody
    • Sly1p antibody
    • STXBP1L2 antibody
    • Syntaxin binding protein 1 like 2 antibody
    • Syntaxin-binding protein 1-like 2 antibody
    • Vesicle transport related protein antibody
    see all

Anti-SCFD1 antibody images

  • All lanes : Anti-SCFD1 antibody (ab86594) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg


    Predicted band size : 72 kDa


    Exposure time : 3 minutes
  • 1mg whole cell lysate from Hela cells was immunoprecipited using 3ug ab86594. 20% of IP was loaded per lane, and probed with ab86549 at 1ug/ml (lane 1) or with a control IgG (lane 2).
    Detection: chemiluminescence with an exposure time of 30 seconds.

References for Anti-SCFD1 antibody (ab86594)

ab86594 has not yet been referenced specifically in any publications.

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