Anti-SCGN/Secretagogin antibody (ab111871)
Key features and details
- Rabbit polyclonal to SCGN/Secretagogin
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SCGN/Secretagogin antibody
See all SCGN/Secretagogin primary antibodies -
Description
Rabbit polyclonal to SCGN/Secretagogin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human SCGN/Secretagogin aa 57-238. (AAH00336)
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Positive control
- HeLa and HCT116 cells and whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab111871 is purified by antigen-affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 32 kDa.
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ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 32 kDa. |
ICC/IF
1/100 - 1/500. |
Target
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Tissue specificity
Expressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells. In the brain, the expression is restricted to distinct subtypes of neurons with highest expression in the molecular layer of the cerebellum (stellate and basket cells), in the anterior part of the pituitary gland, in the thalamus, in the hypothalamus and in a subgroup of neocortical neurons. -
Sequence similarities
Contains 6 EF-hand domains. -
Cellular localization
Cytoplasm. Secreted. Cytoplasmic vesicle > secretory vesicle membrane. Predominantly cytoplasmic. A small proportion is associated with secretory granules and membrane fractions (By similarity). Detectable in human serum after ischemic neuronal damage. - Information by UniProt
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Database links
- Entrez Gene: 618206 Cow
- Entrez Gene: 10590 Human
- Entrez Gene: 214189 Mouse
- Entrez Gene: 306942 Rat
- Omim: 609202 Human
- SwissProt: A5PJN0 Cow
- SwissProt: O76038 Human
- SwissProt: Q91WD9 Mouse
see all -
Alternative names
- Calbindin like antibody
- CALBL antibody
- DJ501N12.8 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab111871 has been referenced in 3 publications.
- Shi Y et al. Mouse and human share conserved transcriptional programs for interneuron development. Science 374:eabj6641 (2021). PubMed: 34882453
- Garas FN et al. Structural and molecular heterogeneity of calretinin-expressing interneurons in the rodent and primate striatum. J Comp Neurol 526:877-898 (2018). Mouse . PubMed: 29218729
- Garas FN et al. Secretagogin expression delineates functionally-specialized populations of striatal parvalbumin-containing interneurons. Elife 5:N/A (2016). PubMed: 27669410