Anti-SCGN antibody - Carboxyterminal end (ab71098)

Overview

  • Product nameAnti-SCGN antibody - Carboxyterminal end
    See all SCGN primary antibodies
  • Description
    Rabbit polyclonal to SCGN - Carboxyterminal end
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) from the C terminal region conjugated to KLH

  • Positive control
    • WB: CEM cell lysate IHC-P: Human lung carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab71098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
ELISA 1/1000.
IHC-P 1/50.

Target

  • Tissue specificityExpressed at high levels in the pancreatic islets of Langerhans and to a much lesser extent in the gastrointestinal tract (stomach, small intestine and colon), the adrenal medulla and cortex and the thyroid C-cells. In the brain, the expression is restricted to distinct subtypes of neurons with highest expression in the molecular layer of the cerebellum (stellate and basket cells), in the anterior part of the pituitary gland, in the thalamus, in the hypothalamus and in a subgroup of neocortical neurons.
  • Sequence similaritiesContains 6 EF-hand domains.
  • Cellular localizationCytoplasm. Secreted. Cytoplasmic vesicle > secretory vesicle membrane. Predominantly cytoplasmic. A small proportion is associated with secretory granules and membrane fractions (By similarity). Detectable in human serum after ischemic neuronal damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • Calbindin like antibody
    • CALBL antibody
    • DJ501N12.8 antibody
    • OTTHUMP00000016124 antibody
    • Scgn antibody
    • SECRET antibody
    • Secretagogin antibody
    • Secretagogin EF hand calcium binding protein antibody
    • SEGN antibody
    • SEGN_HUMAN antibody
    • Setagin antibody
    see all

Anti-SCGN antibody - Carboxyterminal end images

  • Anti-SCGN antibody - Carboxyterminal end (ab71098) at 1/60 dilution + CEM cell lysate at 35 µg

    Predicted band size : 32 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa,56 kDa. We are unsure as to the identity of these extra bands.
  • Formalin fixed and paraffin embedded human lung carcinoma tissue labelled with ab71098 at 1/50 dilution to detect SCGN. ab71098 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

References for Anti-SCGN antibody - Carboxyterminal end (ab71098)

ab71098 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab71098.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"