Overview

  • Product name
    Anti-SCHIP1 antibody
  • Description
    Rabbit polyclonal to SCHIP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SCHIP1 aa 97-126 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9P0W5

  • Positive control
    • HeLa cell line lysates; Human cerebellum tissue; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99.91% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab170587 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 53 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Tissue specificity
    Isoform 5 and isoform 6 are highly expressed in the brain. Both isoforms derive from naturally occurring readthrough transcripts which produce IQCJ-SCHIP1 fusion proteins.
  • Cellular localization
    Cytoplasm. Partially colocalizes with NF2 beneath the cytoplasmic membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ39160 antibody
    • SCHI1_HUMAN antibody
    • SCHIP 1 antibody
    • SCHIP-1 antibody
    • Schip1 antibody
    • schwannomin interacting protein 1 antibody
    • Schwannomin-interacting protein 1 antibody
    see all

Images

  • Flow Cytometric analysis of HeLa cells labeling SCHIP1 with ab170587 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human cerebellum tissue labeling SCHIP1 with ab170587 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Anti-SCHIP1 antibody (ab170587) at 1/100 dilution + HeLa cell line lysates at 35 µg

    Predicted band size: 53 kDa

References

ab170587 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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