Schneider L2 whole cell lysate (ab14893)

Overview

  • Product name
    Schneider L2 whole cell lysate
    See all Schneider L2 lysates
  • General notes
    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.90
    Constituents: 0.48% HEPES, 0.75% Potassium chloride, 0.01% Magnesium chloride, 20% Glycerol, 0.009% PMSF, 0.008% DTT
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Background
    This permanent line is derived from a primary culture of late stage (L2) (20-24 hours old) Drosophila melanogaster embryos (Schneider, 1972) prepared in Scheiders insect medium. It is a positive control for the transcription factor GAGA ab11962.

References

ab14893 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry. I am afraid we have not tested this yet so can't provide further information.

Thank you for your enquiry. The Drosophila SL2 lysate is supplied in 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9. I would advise diluting the lysate in the same buffer and storing in...

Read More

Thank you for your enquiry. May I please apologise for the delay to your question enquiring about the number of cells required to derive 100ug of lysate. The cell lysates were generated in large batches and then aliquoted, 100 ug per vial. Our supp...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up