Overview

  • Product nameAnti-Sclerostin antibody
    See all Sclerostin primary antibodies
  • Description
    Rabbit polyclonal to Sclerostin
  • Tested applicationsWB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human
  • Immunogen

    Synthetic peptide corresponding to Human Sclerostin aa 12-42 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 50964

  • Positive control
    • WB: Mouse liver tissue lysate IHC-P: Human breast carcinoma tissue

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab63097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

  • FunctionNegative regulator of bone growth.
  • Tissue specificityWidely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteeoblasts differentiated for 21 days.
  • Involvement in diseaseDefects in SOST are the cause of sclerosteosis (SOST) [MIM:269500]; also known as cortical hyperostosis with syndactyly. SOST is an autosomal recessive sclerosing bone dysplasia characterized by a generalized hyperostosis and sclerosis leading to a markedly thickened skull, with mandible, ribs, clavicles and all long bones also being affected. Due to narrowing of the foramina of the cranial nerves, facial nerve palsy, hearing loss and atrophy of the optic nerves can occur. Sclerosteosis is clinically and radiologically very similar to van Buchem disease, mainly differentiated by hand malformations and a large stature in sclerosteosis patients.
    Note=A 52 kb deletion downstream of SOST results in SOST transcription suppression and is a cause of van Buchem disease (VBCH) [MIM:239100]; also known as hyperostosis corticalis generalisata. VBCH is an autosomal recessive sclerosing bone dysplasia characterized by endosteal hyperostosis of the mandible, skull, ribs, clavicles, and diaphyses of the long bones. Affected patients present a symmetrically increased thickness of bones, most frequently found as an enlarged jawbone, but also an enlargement of the skull, ribs, diaphysis of long bones, as well as tubular bones of hands and feet. The clinical consequence of increased thickness of the skull include facial nerve palsy causing hearing loss, visual problems, neurological pain, and, very rarely, blindness as a consequence of optic atrophy. Serum alkaline phosphatase levels are elevated.
  • Sequence similaritiesBelongs to the sclerostin family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BEER antibody
    • CDD antibody
    • Cortical hyperostosis with syndactyly antibody
    • Sclerosteosis antibody
    • Sclerostin antibody
    • SOST antibody
    • SOST_HUMAN antibody
    • UNQ2976/PRO7455/PRO7476 antibody
    • VBCH antibody
    see all

Anti-Sclerostin antibody images

  • Anti-Sclerostin antibody (ab63097) at 1/500 dilution + Rat bone (tibia) whole tissue lysate at 36 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97069) at 1/2000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : 55 kDa (possible non-specific binding),60 kDa (possible non-specific binding).

    Exposure time : 10 minutes

    This image is courtesy of an Abreview submitted by Helder Fonseca

    See Abreview

  • Anti-Sclerostin antibody (ab63097) at 1/60 dilution + mouse liver tissue lysates at 35 µg

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical analysis of sclerostin expression in paraffin embedded formalin fixed human breast carcinoma tissue section using 1/10 ab63097, followed by HRP conjugated secondary antibody step and DAB staining.

References for Anti-Sclerostin antibody (ab63097)

This product has been referenced in:
  • Cohen-Kfir E  et al. Sirt1 is a regulator of bone mass and a repressor of Sost encoding for sclerostin, a bone formation inhibitor. Endocrinology 152:4514-24 (2011). Mouse . Read more (PubMed: 21952235) »
  • Mardaryev AN  et al. Micro-RNA-31 controls hair cycle-associated changes in gene expression programs of the skin and hair follicle. FASEB J 24:3869-81 (2010). WB ; Mouse . Read more (PubMed: 20522784) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 36 µg
Gel Running Conditions Reduced Denaturing (10 %)
Sample Rat Tissue lysate - whole (Bone - Tibia)
Specification Bone - Tibia
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Mr. Helder Fonseca

Verified customer

Submitted Sep 17 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (SW1353 human chondrosacroma cell line)
Loading amount 40 µg
Specification SW1353 human chondrosacroma cell line
Treatment Transfected with full length human SOST
Gel Running Conditions Reduced Denaturing (10% Bis-Tris Gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 11 2013

Thank you for contacting Abcam.

The western blot image for ab63097 was provided by the supplying laboratory of this product. The antibody was not one that was produced by Abcam, however we are confident about the data that is shown on our web...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (Bone and cartilage)
Specification Bone and cartilage
Fixative 10% neutral-buffered formalin
Antigen retrieval step None
Permeabilization No
Blocking step Protein-Block Serum Free (DakoCytomation) as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted May 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"