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Synthetic peptide corresponding to Human Sclerostin aa 12-42 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: 50964
Our Abpromise guarantee covers the use of ab63097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).|
|IHC-P||1/10 - 1/50.|
This image is courtesy of an Abreview submitted by Helder Fonseca
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Sclerostin with ab63097. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"