Overview

  • Product name
    Anti-SCMH1 antibody
  • Description
    Rabbit polyclonal to SCMH1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 438-660 of Human SCMH1.

  • Positive control
    • IMR32 and Mouse brain whole cell lysates. HeLa whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 73 kDa.
IP 1/500 - 1/1000.

Target

  • Function
    Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
  • Tissue specificity
    Strongly expressed in heart, muscle and pancreas. Weakly expressed in brain, placenta, lung, liver and kidney.
  • Sequence similarities
    Belongs to the SCM family.
    Contains 2 MBT repeats.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Polycomb protein SCMH1 antibody
    • SCMH 1 antibody
    • SCMH1 antibody
    • SCMH1_HUMAN antibody
    • Scml 3 antibody
    • Scml3 antibody
    • Sex comb on midleg 1 antibody
    • Sex comb on midleg homolog 1 antibody
    see all

Images

  • Anti-SCMH1 antibody (ab153937) at 1/5000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size: 73 kDa



    7.5% SDS PAGE
  • Anti-SCMH1 antibody (ab153937) at 1/10000 dilution + Mouse brain whole cell lysate at 20 µg

    Predicted band size: 73 kDa



    7.5% SDS PAGE
  • Immunoprecipitation of SCMH1 in HeLa whole cell lysate using 2.5µg ab153937.
    Lane 1: 40 µg of HeLa whole cell lysate.
    Lane 2: Control with 2.5 µg of preimmune rabbit IgG.
    Lane 3: Immunoprecipitation of SCMH1 protein.
    Western blot detection with ab153937 at 1/1000 dilution.
    7.5% SDS-PAGE.

References

ab153937 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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