Overview

  • Product nameAnti-SCMH1 antibody
    See all SCMH1 primary antibodies
  • Description
    Mouse monoclonal to SCMH1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus Monkey
  • Immunogen

    Recombinant fragment: EKLCHNLRSD NLFGNQPFTQ THLSLTAIEY SHSHDRYLPG ETFVLGNSLA RSLEPHSDSM DSASNPTNLV STSQRHRPLL SSCGLPPSTA SAVRRLCSR, corresponding to amino acids 404-502 of Human SCMH1 (NP_036368) with proprietory tag.

  • Positive control
    • HepG2 cell lysate. Recombinant tagged human SCMH1 fragment.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab77648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 73 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged SCMH1 is approximately 30ng/ml when used as a capture antibody.

Target

  • FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
  • Tissue specificityStrongly expressed in heart, muscle and pancreas. Weakly expressed in brain, placenta, lung, liver and kidney.
  • Sequence similaritiesBelongs to the SCM family.
    Contains 2 MBT repeats.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Polycomb protein SCMH1 antibody
    • SCMH 1 antibody
    • SCMH1 antibody
    • SCMH1_HUMAN antibody
    • Scml 3 antibody
    • Scml3 antibody
    • Sex comb on midleg 1 antibody
    • Sex comb on midleg homolog 1 antibody
    see all

Anti-SCMH1 antibody images

  • Anti-SCMH1 antibody (ab77648) at 1 µg/ml + Recombinant tagged human SCMH1 fragment at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 73 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-SCMH1 antibody (ab77648) at 1 µg/ml + HepG2 cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 73 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

References for Anti-SCMH1 antibody (ab77648)

ab77648 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"