Overview

  • Product nameAnti-SCML1 antibody
    See all SCML1 primary antibodies
  • Description
    Rabbit polyclonal to SCML1
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig, Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (ESYSPTLPVS RRENNSPSNL PRPSFCMEEY QRAELEEDPI LSRTPSPVHP) of Human SCML1 (NP_006737).

  • Positive control
    • HeLa cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab83665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/12500.

Target

  • FunctionPutative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. May be involved in spermatogenesis during sexual maturation.
  • Tissue specificityUbiquitous. Expressed in fetal and adult tissues.
  • Sequence similaritiesBelongs to the SCM family.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gm1792 antibody
    • Gm47 antibody
    • OTTHUMP00000022996 antibody
    • OTTHUMP00000025692 antibody
    • RP3-389A20.1 antibody
    • SCML1 antibody
    • SCML1_HUMAN antibody
    • Sex comb on midleg (Drosophila) like 1 antibody
    • Sex comb on midleg like 1 (Drosophila) antibody
    • Sex comb on midleg like 1 antibody
    • Sex comb on midleg like protein 1 antibody
    • Sex comb on midleg-like protein 1 antibody
    • Vsex comb on midleg like 1 antibody
    see all

Anti-SCML1 antibody images

  • Anti-SCML1 antibody (ab83665) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling SCML1 with ab83665 at 5.0µg/ml.

References for Anti-SCML1 antibody (ab83665)

ab83665 has not yet been referenced specifically in any publications.

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