Overview

  • Product name
  • Description
    Rabbit polyclonal to SCML2
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 650-700 of Human SCML2 (Q9UQR0).

  • Positive control
    • HeLa cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab115160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 77 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development.
  • Tissue specificity
    Highly expressed in placenta, thymus and testis. Detected at lower levels in brain, liver, skeletal muscle, pancreas and ovary.
  • Sequence similarities
    Belongs to the SCM family.
    Contains 2 MBT repeats.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • SCML2 antibody
    • SCML2_HUMAN antibody
    • sex comb on midleg like 2 (Drosophila) antibody
    • Sex comb on midleg like 2 antibody
    • Sex comb on midleg like protein 2 antibody
    • Sex comb on midleg, Drosophila, homolog-like 2 antibody
    • Sex comb on midleg-like protein 2 antibody
    see all

Anti-SCML2 antibody images

  • All lanes : Anti-SCML2 antibody (ab115160) at 0.1 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : 293T cell lysate at 50 µg
    Lane 4 : Jurkat cell lysate at 50 µg


    Predicted band size : 77 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation of SCML2 (6 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with an anti-SCML2 antibody that recognizes an upstream epitope (ab115157); Lane 2-Immunoprecipitation with anti-SCML2 antibody (ab115160); Lane 3- Immunoprecipitation with IgG control. Anti-SCML2 antibody (ab115160) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 30 sec.

References for Anti-SCML2 antibody (ab115160)

ab115160 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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