Anti-SCML2 antibody [SCMAD14A] (ab51506)
Key features and details
- Mouse monoclonal [SCMAD14A] to SCML2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-SCML2 antibody [SCMAD14A] -
Description
Mouse monoclonal [SCMAD14A] to SCML2 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, C terminal. (Human)
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Positive control
- HeLa Whole cell lysate, NIH3T3 whole cell lysate, and F2408 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab51506 purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
Clonality
Monoclonal -
Clone number
SCMAD14A -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/200. Detects a band of approximately 100 kDa (predicted molecular weight: 77.3 kDa).
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Notes |
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WB
1/200. Detects a band of approximately 100 kDa (predicted molecular weight: 77.3 kDa). |
Target
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Function
Putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. -
Tissue specificity
Highly expressed in placenta, thymus and testis. Detected at lower levels in brain, liver, skeletal muscle, pancreas and ovary. -
Sequence similarities
Belongs to the SCM family.
Contains 2 MBT repeats.
Contains 1 SAM (sterile alpha motif) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10389 Human
- Entrez Gene: 107815 Mouse
- Entrez Gene: 367793 Rat
- Omim: 300208 Human
- SwissProt: Q9UQR0 Human
- Unigene: 495774 Human
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Alternative names
- SCML2 antibody
- SCML2_HUMAN antibody
- sex comb on midleg like 2 (Drosophila) antibody
see all
Images
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Anti-SCML2 antibody [SCMAD14A] (ab51506) at 1/200 dilution + HeLa whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 77.3 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Same results obtained using NIH3T3 whole cell lysate, and F2408 whole cell lysate.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab51506 has been referenced in 1 publication.
- Galoian K et al. Epigenetic regulation of embryonic stem cell marker miR302C in human chondrosarcoma as determinant of antiproliferative activity of proline-rich polypeptide 1. Int J Oncol 47:465-72 (2015). PubMed: 26094604