Overview

  • Product name
    Anti-SCML2 antibody [SCMAD14A]
    See all SCML2 primary antibodies
  • Description
    Mouse monoclonal [SCMAD14A] to SCML2
  • Tested applications
    Suitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, C terminal. (Human)

  • Positive control
    • HeLa Whole cell lysate, NIH3T3 whole cell lysate, and F2408 whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, 8mM Dibasic monohydrogen sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Monobasic dihydrogen potassium phosphate, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab51506 purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • Clonality
    Monoclonal
  • Clone number
    SCMAD14A
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Detects a band of approximately 100 kDa (predicted molecular weight: 77.3 kDa).
Dot blot Use at an assay dependent dilution.

Target

  • Function
    Putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development.
  • Tissue specificity
    Highly expressed in placenta, thymus and testis. Detected at lower levels in brain, liver, skeletal muscle, pancreas and ovary.
  • Sequence similarities
    Belongs to the SCM family.
    Contains 2 MBT repeats.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • SCML2 antibody
    • SCML2_HUMAN antibody
    • sex comb on midleg like 2 (Drosophila) antibody
    • Sex comb on midleg like 2 antibody
    • Sex comb on midleg like protein 2 antibody
    • Sex comb on midleg, Drosophila, homolog-like 2 antibody
    • Sex comb on midleg-like protein 2 antibody
    see all

Images

  • Anti-SCML2 antibody [SCMAD14A] (ab51506) at 1/200 dilution + HeLa whole cell lysate at 25 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size : 77.3 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Galoian K  et al. Epigenetic regulation of embryonic stem cell marker miR302C in human chondrosarcoma as determinant of antiproliferative activity of proline-rich polypeptide 1. Int J Oncol 47:465-72 (2015). Read more (PubMed: 26094604) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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