Purification notesab51506 purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200. Detects a band of approximately 100 kDa (predicted molecular weight: 77.3 kDa).
Use at an assay dependent dilution.
FunctionPutative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development.
Tissue specificityHighly expressed in placenta, thymus and testis. Detected at lower levels in brain, liver, skeletal muscle, pancreas and ovary.
Sequence similaritiesBelongs to the SCM family. Contains 2 MBT repeats. Contains 1 SAM (sterile alpha motif) domain.