Overview

  • Product name
  • Description
    Rabbit polyclonal to SCML4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-237 of Human SCML4 (UniProt ID: Q8N228).

  • Positive control
    • SK N SH whole cell lysate; BT483 xenograft tissue; C2C12 xenograft tissue.
  • General notes
    Store as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    SCML4 is a putative Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • dJ47M23.1 antibody
    • FLJ36252 antibody
    • Sex comb on midleg like 4 (Drosophila) antibody
    • Sex comb on midleg like 4 antibody
    • Sex comb on midleg like 4 Drosophila antibody
    • Sex comb on midleg like protein 4 antibody
    see all

Images

  • Anti-SCML4 antibody (ab154011) at 1/1000 dilution + SK-N-SH whole cell lysate at 30 µg

    Predicted band size: 45 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded BT483 xenograft tissue labeling SCML4 with ab154011 at 1/750 dilution.
  • Immunohistochemical analysis of paraffin embedded C2C12 xenograft tissue, labeling SCML4 with ab154011 at 1/750 dilution.

References

ab154011 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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