Anti-Scn1a antibody [S74-71] (ab106514)

Overview

  • Product nameAnti-Scn1a antibody [S74-71]
    See all Scn1a primary antibodies
  • Description
    Mouse monoclonal [S74-71] to Scn1a
  • SpecificityNo cross reactivity against Scn2a, Scn3a and Scn8a.
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein, corresponding to cytoplasmic C-terminal amino acids 1929-2009 of Rat Scn1a (P04774).

  • Positive control
    • Rat brain lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberS74-71
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 229 kDa.
IHC-P Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
ICC Use a concentration of 0.1 - 1 µg/ml.
ICC/IF Use a concentration of 1 - 10 µg/ml.

Target

  • FunctionMediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.
  • Involvement in diseaseDefects in SCN1A are the cause of generalized epilepsy with febrile seizures plus type 2 (GEFS+2) [MIM:604233]. Generalized epilepsy with febrile seizures-plus refers to a rare autosomal dominant, familial condition with incomplete penetrance and large intrafamilial variability. Patients display febrile seizures persisting sometimes beyond the age of 6 years and/or a variety of afebrile seizure types. GEFS+ is a disease combining febrile seizures, generalized seizures often precipitated by fever at age 6 years or more, and partial seizures, with a variable degree of severity.
    Defects in SCN1A are a cause of severe myoclonic epilepsy in infancy (SMEI) [MIM:607208]; also called Dravet syndrome. SMEI is a rare disorder characterized by generalized tonic, clonic, and tonic-clonic seizures that are initially induced by fever and begin during the first year of life. Later, patients also manifest other seizure types, including absence, myoclonic, and simple and complex partial seizures. Psychomotor development delay is observed around the second year of life. SMEI is considered to be the most severe phenotype within the spectrum of generalized epilepsies with febrile seizures-plus.
    Defects in SCN1A are a cause of intractable childhood epilepsy with generalized tonic-clonic seizures (ICEGTC) [MIM:607208]. ICEGTC is a disorder characterized by generalized tonic-clonic seizures beginning usually in infancy and induced by fever. Seizures are associated with subsequent mental decline, as well as ataxia or hypotonia. ICEGTC is similar to SMEI, except for the absence of myoclonic seizures.
    Defects in SCN1A are the cause of migraine familial hemiplegic type 3 (FHM3) [MIM:609634]. FHM3 is an autosomal dominant severe subtype of migraine with aura characterized by some degree of hemiparesis during the attacks. The episodes are associated with variable features of nausea, vomiting, photophobia, and phonophobia. Age at onset ranges from 6 to 15 years. FHM is occasionally associated with other neurologic symptoms such as cerebellar ataxia or epileptic seizures. A unique eye phenotype of elicited repetitive daily blindness has also been reported to be cosegregating with FHM in a single Swiss family.
    Defects in SCN1A are the cause of familial febrile convulsions type 3A (FEB3A) [MIM:604403]; also known as familial febrile seizures 3. Febrile convulsions are seizures associated with febrile episodes in childhood without any evidence of intracranial infection or defined pathologic or traumatic cause. It is a common condition, affecting 2-5% of children aged 3 months to 5 years. The majority are simple febrile seizures (generally defined as generalized onset, single seizures with a duration of less than 30 minutes). Complex febrile seizures are characterized by focal onset, duration greater than 30 minutes, and/or more than one seizure in a 24 hour period. The likelihood of developing epilepsy following simple febrile seizures is low. Complex febrile seizures are associated with a moderately increased incidence of epilepsy.
  • Sequence similaritiesBelongs to the sodium channel (TC 1.A.1.10) family. Nav1.1/SCN1A subfamily.
    Contains 1 IQ domain.
  • DomainThe sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • brain sodium channel type I antibody
    • EIEE6 antibody
    • FEB3 antibody
    • FEB3A antibody
    • FHM3 antibody
    • GEFS+2 antibody
    • GEFSP2 antibody
    • HBSC I antibody
    • HBSCI antibody
    • NAC1 antibody
    • Nav 1.1 antibody
    • RBI antibody
    • SCN1 antibody
    • Scn1a antibody
    • SCN1A_HUMAN antibody
    • SMEI antibody
    • Sodium channel protein brain I alpha subunit antibody
    • Sodium channel protein brain I subunit alpha antibody
    • Sodium channel protein type 1 subunit alpha antibody
    • Sodium channel protein type I subunit alpha antibody
    • Sodium channel voltage gated type 1 alpha subunit antibody
    • Sodium channel voltage gated type I alpha polypeptide antibody
    • Voltage-gated sodium channel subunit alpha Nav1.1 antibody
    see all

Anti-Scn1a antibody [S74-71] images

  • Immunohistochemical detection of Scn1a in frozen sections of Mouse brain extract using ab106514.

References for Anti-Scn1a antibody [S74-71] (ab106514)

ab106514 has not yet been referenced specifically in any publications.

Product Wall

I'm happy to help! Please let me know if you have any further questions.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab24820.
To check the status of the order please contact our Cus...

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Thanks for your email.
I am sorry to hear you are still having trouble using ab106514 to detect Nav1.1. As you are having success detecting the target using a different antibody, it does seem that the vial of ab106514 you have received is not work...

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Thank you for your enquiry.
We don't have a specific protocol for immunostaining to be used with ab106514. Here is a link to a good general ICC protocol on our website:
http://www.abcam.com/index.html?pageconfig=resource&rid=11417
I hope...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"