Overview

  • Product name
  • Description
    Rabbit polyclonal to SCN3A
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human SCN3A aa 80-130.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
WB 1/1000 - 1/5000. Predicted molecular weight: 226 kDa.

Target

  • Function
    Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.
  • Sequence similarities
    Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.3/SCN3A subfamily.
    Contains 1 IQ domain.
  • Domain
    The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
  • Post-translational
    modifications
    May be ubiquitinated by NEDD4L; which would promote its endocytosis.
    Phosphorylation at Ser-1501 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • brain III voltage-gated sodium channel antibody
    • NAC3 antibody
    • Nav1.3 antibody
    • SCN3A antibody
    • SCN3A_HUMAN antibody
    • Sodium channel protein brain III subunit alpha antibody
    • Sodium channel protein type 3 subunit alpha antibody
    • Sodium channel protein type III subunit alpha antibody
    • Sodium channel protein, brain III subunit alpha antibody
    • sodium channel, brain type III, alpha subunit antibody
    • sodium channel, neuronal type III, alpha subunit antibody
    • sodium channel, voltage-gated, type III, alpha polypeptide antibody
    • sodium channel, voltage-gated, type III, alpha subunit antibody
    • Voltage gated sodium channel subtype III antibody
    • Voltage gated sodium channel subunit alpha Nav1.3 antibody
    • Voltage-gated sodium channel subtype III antibody
    • Voltage-gated sodium channel subunit alpha Nav1.3 antibody
    see all

Images

  • ICC/IF image of ab65164 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65164, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab65164 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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