This fast track antibody is not yet fully characterized. It is subject to these terms and conditions


  • Product name
  • Description
    Rabbit polyclonal to SCN4B

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Host species
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human SCN4B.

    (Peptide available as ab95413.)

  • Positive control
    • Brain (Human) Tissue Lysate; Brain (Rat) Tissue Lysate ; U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate ; Spinal Cord (Human) Tissue Lysate; Spinal Cord (Rat) Tissue Lysate ; Skeletal Muscle (Human) Tissue Lysate


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


  • Relevance
    The SCN4B protein modulates channel gating kinetics. Causes negative shifts in the voltage dependence of activation of certain alpha sodium channels, but does not affect the voltage dependence of inactivation By similarity. The protein has been found to be expressed at a high level in dorsal root ganglia, at a lower level in brain, spinal cord, skeletal muscle and heart. Defects in SCN4B are the cause of long QT syndrome type 10 (LQT10) [MIM:611819]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy.
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • SCN4B_HUMAN antibody
    • Sodium channel subunit beta-4 antibody


This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • All lanes : Anti-SCN4B antibody (ab80539) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 4 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 5 : Spinal Cord (Rat) Tissue Lysate
    Lane 6 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg/ml per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 20 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 8 minutes

    Further characterization on this product is underway. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. SCN4B contains at least three potential glycosylation sites (SwissProt), which may explain its migration at a higher molecular weight than predicted. The primary sequence contains a 30-residue signal sequence at the N-terminus. The band around 20-kDa in human skeletal muscle may be the unglycosylated form of the target protein.
  • ICC/IF image of ab80539 stained PC12 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80539, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Barbosa C  et al. Navß4 regulates fast resurgent sodium currents and excitability in sensory neurons. Mol Pain 11:60 (2015). Read more (PubMed: 26408173) »
  • Tan ZY  et al. Tetrodotoxin-resistant sodium channels in sensory neurons generate slow resurgent currents that are enhanced by inflammatory mediators. J Neurosci 34:7190-7 (2014). Read more (PubMed: 24849353) »

See all 2 Publications for this product

Customer reviews and Q&As

Western blot
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jul 10 2015

Western blot
Loading amount
40 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (9% acrylamide)
Rat Tissue lysate - other (muscle)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Mr. Baptiste Jude

Verified customer

Submitted Feb 05 2015


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