This fast track antibody is not yet fully characterized. It is subject to these terms and conditions


  • Product nameAnti-SCN4B antibody
    See all SCN4B primary antibodies
  • Description
    Rabbit polyclonal to SCN4B

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human SCN4B.

    (Peptide available as ab95413.)

  • Positive control
    • Brain (Human) Tissue Lysate; Brain (Rat) Tissue Lysate ; U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate ; Spinal Cord (Human) Tissue Lysate; Spinal Cord (Rat) Tissue Lysate ; Skeletal Muscle (Human) Tissue Lysate


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


  • RelevanceThe SCN4B protein modulates channel gating kinetics. Causes negative shifts in the voltage dependence of activation of certain alpha sodium channels, but does not affect the voltage dependence of inactivation By similarity. The protein has been found to be expressed at a high level in dorsal root ganglia, at a lower level in brain, spinal cord, skeletal muscle and heart. Defects in SCN4B are the cause of long QT syndrome type 10 (LQT10) [MIM:611819]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy.
  • Cellular localizationCell Membrane
  • Database links
  • Alternative names
    • SCN4B_HUMAN antibody
    • Sodium channel subunit beta-4 antibody

Anti-SCN4B antibody images

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • All lanes : Anti-SCN4B antibody (ab80539) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 4 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 5 : Spinal Cord (Rat) Tissue Lysate
    Lane 6 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg/ml per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ICC/IF image of ab80539 stained PC12 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80539, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-SCN4B antibody (ab80539)

This product has been referenced in:
  • Barbosa C  et al. Navß4 regulates fast resurgent sodium currents and excitability in sensory neurons. Mol Pain 11:60 (2015). Read more (PubMed: 26408173) »
  • Tan ZY  et al. Tetrodotoxin-resistant sodium channels in sensory neurons generate slow resurgent currents that are enhanced by inflammatory mediators. J Neurosci 34:7190-7 (2014). Read more (PubMed: 24849353) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Brain)
Gel Running Conditions Reduced Denaturing
Loading amount 40 µg
Specification Brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jul 10 2015

Application Western blot
Loading amount 40 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (9% acrylamide)
Sample Rat Tissue lysate - other (muscle)
Specification muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Mr. Baptiste Jude

Verified customer

Submitted Feb 05 2015