The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: Use at an assay dependent dilution (pmid 19607678).
IHC-P: Use at an assay dependent dilution.
WB: 1/500 - 1/5000. Predicted molecular weight: 193 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceVoltage dependent sodium channels mediate the sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, they form a sodium selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. These ion channel proteins exist as heteromultimeric complexes of a large alpha subunit and 1 or 2 smaller beta subunits. SCN7A (Sodium channel protein type 7 subunit alpha) is an alpha subunit and is expressed in heart and uterus.
IHC image of SCN7A staining in Human normal heart muscle formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab66499, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Immunocytochemistry/ Immunofluorescence - Anti-SCN7A antibody (ab66499)Image from Pinto FM et al., Reprod Biol Endocrinol. 2009 Jul 16;7:71. Fig 2.; doi:10.1186/1477-7827-7-71; 16 July, 2009
ab66499 staining SCN7A in Human sperm cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with methanol and blocked with 2% BSA in PBS for 30 min. Samples were incubated with primary antibody (1/100 in 2% BSA in PBS) overnight at 4°C. A FITC-conjugated anti-rabbit IgG was used as the secondary antibody.
References for Anti-SCN7A antibody (ab66499)
This product has been referenced in:
Lara LS et al. The sodium-activated sodium channel is expressed in the rat kidney thick ascending limb and collecting duct cells and is upregulated during high salt intake. Am J Physiol Renal Physiol303:F105-9 (2012).
Read more (PubMed: 22442212) »
Ke CB et al. Enhanced SCN7A/Nax expression contributes to bone cancer pain by increasing excitability of neurons in dorsal root ganglion. Neuroscience227C:80-89 (2012).
Read more (PubMed: 23026072) »