Overview

  • Product name
    Anti-SCN7A antibody
  • Description
    Rabbit polyclonal to SCN7A
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from the C terminal domain of human SCNA7.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: Whole serum
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Lots GR325281-1 and GR325281-1 are whole antiserum.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 19607678
WB 1/500 - 1/5000. Predicted molecular weight: 193 kDa.
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    Voltage dependent sodium channels mediate the sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, they form a sodium selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. These ion channel proteins exist as heteromultimeric complexes of a large alpha subunit and 1 or 2 smaller beta subunits. SCN7A (Sodium channel protein type 7 subunit alpha) is an alpha subunit and is expressed in heart and uterus.
  • Cellular localization
    Membrane; Multipass membrane protein.
  • Database links
  • Alternative names
    • Putative voltage gated sodium channel subunit alpha Nax antibody
    • SCN 6A antibody
    • SCN 7A antibody
    • SCN6A antibody
    • Sodium channel protein cardiac and skeletal muscle subunit alpha antibody
    • Sodium channel protein type 7 subunit alpha antibody
    • Sodium channel protein type VII subunit alpha antibody
    • Sodium channel voltage gated type VI alpha polypeptide antibody
    • Sodium channel voltage gated type VII alpha antibody
    • Sodium channel voltage gated type VII alpha polypeptide antibody
    • Voltage dependent sodium channel alpha subunit antibody
    see all

Images

  • ab66499 staining SCN7A in Human sperm cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with methanol and blocked with 2% BSA in PBS for 30 min. Samples were incubated with primary antibody (1/100 in 2% BSA in PBS) overnight at 4°C. A FITC-conjugated anti-rabbit IgG was used as the secondary antibody.

References

This product has been referenced in:
  • Lara LS  et al. The sodium-activated sodium channel is expressed in the rat kidney thick ascending limb and collecting duct cells and is upregulated during high salt intake. Am J Physiol Renal Physiol 303:F105-9 (2012). WB ; Rat . Read more (PubMed: 22442212) »
  • Ke CB  et al. Enhanced SCN7A/Nax expression contributes to bone cancer pain by increasing excitability of neurons in dorsal root ganglion. Neuroscience 227C:80-89 (2012). Read more (PubMed: 23026072) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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