Overview

  • Product name
  • Description
    Rabbit polyclonal to SCNN1B
  • Specificity
    Detects recombinant rat beta-epithelial sodium channel (SCNN1B).
  • Tested applications
    Suitable for: IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Rabbit
  • Immunogen

    Synthetic peptide corresponding to Human SCNN1B aa 619-640.
    Sequence:

    NYDSLRLQPLDVIESDSEGDAI


    (Peptide available as ab4926)

Properties

Applications

Our Abpromise guarantee covers the use of ab2906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

By immunoprecipitation this antibody specifically binds to unglycosylated, ~75 kDa recombinant rat SCNN1B protein.

IHC-P Use at an assay dependent concentration.

Target

  • Function
    Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
  • Involvement in disease
    Pseudohypoaldosteronism 1, autosomal recessive
    Liddle syndrome
    Bronchiectasis with or without elevated sweat chloride 1
  • Sequence similarities
    Belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family. SCNN1B subfamily.
  • Post-translational
    modifications
    Phosphorylated on serine and threonine residues.
  • Cellular localization
    Apical cell membrane. Apical membrane of epithelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amiloride sensitive sodium channel subunit beta antibody
    • Amiloride-sensitive sodium channel subunit beta antibody
    • Beta NaCH antibody
    • Beta-ENaC antibody
    • Beta-NaCH antibody
    • ENaC beta antibody
    • ENaCB antibody
    • Epithelial Na(+) channel subunit beta antibody
    • Epithelial Na+ channel beta subunit antibody
    • Epithelial Na+ channel subunit beta antibody
    • Epithelial sodium channel beta 2 subunit antibody
    • Epithelial sodium channel beta 3 subunit antibody
    • Nonvoltage gated sodium channel 1 beta subunit antibody
    • Nonvoltage gated sodium channel 1 subunit beta antibody
    • Nonvoltage-gated sodium channel 1 subunit beta antibody
    • SCNEB antibody
    • SCNN 1B antibody
    • SCNN1B antibody
    • SCNNB_HUMAN antibody
    • Sodium channel nonvoltage gated 1 beta (Liddle syndrome) antibody
    • Sodium channel nonvoltage gated 1 beta antibody
    see all

References

This product has been referenced in:
  • Yusef YR  et al. Estrogen increases ENaC activity via PKCd signaling in renal cortical collecting duct cells. Physiol Rep 2:N/A (2014). WB . Read more (PubMed: 24872356) »
  • Zhou Y  et al. Induction of renal 20-hydroxyeicosatetraenoic acid by clofibrate attenuates high-fat diet-induced hypertension in rats. J Pharmacol Exp Ther 317:11-8 (2006). Read more (PubMed: 16339392) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
40 µg
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

Submitted Apr 24 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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