Overview

  • Product nameAnti-SCO2 antibody
    See all SCO2 primary antibodies
  • Description
    Rabbit polyclonal to SCO2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human SCO2.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HEK293; MCF7; MDA MB 231; A431; A549; Caco2.

Properties

Applications

Our Abpromise guarantee covers the use of ab113758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 29 kDa).

Target

  • FunctionActs as a copper chaperone, transporting copper to the Cu(A) site on the cytochrome c oxidase subunit II (COX2).
  • Tissue specificityUbiquitous.
  • Involvement in diseaseDefects in SCO2 are the cause of fatal infantile cardioencephalomyopathy with cytochrome c oxidase deficiency (FIC) [MIM:604377]. This disease is characterized by hypertrophic cardiomyopathy, lactic acidosis, and gliosis. Heart and skeletal muscle show reductions in cytochrome c oxidase (COX) activity, whereas liver and fibroblasts show mild COX deficiencies.
  • Sequence similaritiesBelongs to the SCO1/2 family.
    Contains 1 thioredoxin domain.
  • Cellular localizationMitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytochrome oxidase deficient homolog 2 antibody
    • MGC125823 antibody
    • MGC125825 antibody
    • OTTHUMP00000196774 antibody
    • OTTHUMP00000196775 antibody
    • Protein SCO2 homolog, mitochondrial antibody
    • SCO (cytochrome oxidase deficient, yeast) homolog 2 antibody
    • SCO 1L antibody
    • SCO 2 antibody
    • SCO cytochrome oxidase deficient homolog 2 (yeast) antibody
    • SCO cytochrome oxidase deficient homolog 2 antibody
    • SCO1L antibody
    • SCO2 antibody
    • SCO2_HUMAN antibody
    • Synthesis of cytochrome c oxidase 2 antibody
    see all

Anti-SCO2 antibody images

  • All lanes : Anti-SCO2 antibody (ab113758) at 1 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 6 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa,56 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References for Anti-SCO2 antibody (ab113758)

ab113758 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"