The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/5000.
Use a concentration of 1 µg/ml. Predicted molecular weight: 44 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
RelevanceScpA (Staphylococcal cysteine proteinase A) is able to degrade elastin. ScpA (Staphylococcal cysteine proteinase A) has a broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate. Prematurely activated/folded staphopain A is inhibited by staphostatin A (scpB), which is probably required to protect staphylococcal cytoplasmic proteins from degradation by scpA. In the cytoplasm, prematurely activated/folded scpA forms a stable non-covalent complex with scpB. Cleavage leads to the activation of scpA probably by an auto-catalytic manner. The catalytic maturation of scpA appears to reside outside the cascade of activation started by the metalloprotease aureolysin (aur) scpA (Staphylococcal cysteine proteinaseA) belongs to the peptidase C47 family.