Anti-Scramblase 1 antibody [13A6 [TEC-23]] (ab77122)

Overview

  • Product nameAnti-Scramblase 1 antibody [13A6 [TEC-23]]
    See all Scramblase 1 primary antibodies
  • Description
    Mouse monoclonal [13A6 [TEC-23]] to Scramblase 1
  • Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Pooled lipid rafts isolated from RBL-2H3 cells.

  • Positive control
    • Rat basophilic leukemia (RBL) cells and cell lysate. RBL lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionMay mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.
    May play a role in the antiviral response of interferon (IFN) by amplifying and enhancing the IFN response through increased expression of select subset of potent antiviral genes. May contribute to cytokine-regulated cell proliferation and differentiation.
  • Tissue specificityExpressed in platelets, erythrocyte membranes, lymphocytes, spleen, thymus, prostate, testis, uterus, intestine, colon, heart, placenta, lung, liver, kidney and pancreas. Not detected in brain and skeletal muscle.
  • Sequence similaritiesBelongs to the phospholipid scramblase family.
  • Post-translational
    modifications
    Known to be palmitoylated at one, yet undefined, site.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ca(2+) dependent phospholipid scramblase 1 antibody
    • Ca(2+)-dependent phospholipid scramblase 1 antibody
    • Erythrocyte phospholipid scramblase antibody
    • MM1 cell-derived transplantability-associated gene 1b, mouse, homolog of antibody
    • MmTRA1a antibody
    • MmTRA1b antibody
    • Nor1 antibody
    • Phospholipid scramblase 1 antibody
    • PL scramblase 1 antibody
    • PLS1_HUMAN antibody
    • PLSCR 1 antibody
    • Plscr1 antibody
    • Scramblase1 antibody
    • Tra1 antibody
    • Tra1a antibody
    • Tra1b antibody
    • Transplantability-associated protein 1 antibody
    • Tras1 antibody
    • Tras2 antibody
    see all

Anti-Scramblase 1 antibody [13A6 [TEC-23]] images

  • ab77122 staining Scramblase 1 in rat basophilic leukemia (RBL) cell line by Immunofluorescence.
  • All lanes : Anti-Scramblase 1 antibody [13A6 [TEC-23]] (ab77122)

    Lane 1 : Rat basophilic leukemia (RBL) cell line lysate
    Lane 2 : Scramblase 1 immunoprecipitate from RBL lysate


    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Scramblase 1 antibody [13A6 [TEC-23]] (ab77122)

This product has been referenced in:
  • Smrz D  et al. Engagement of phospholipid scramblase 1 in activated cells: implication for phosphatidylserine externalization and exocytosis. J Biol Chem 283:10904-18 (2008). Read more (PubMed: 18281686) »

See 1 Publication for this product

Product Wall

The recommended working concentration of 1 ug/ml on the WB image was for an old purified batch of Anti-Scramblase 1 antibody [13A6 [TEC-23]]. However, our current batches are in ascites format (concentration not determined), as purification of this par...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"