Overview

  • Product nameAnti-Scratch 2 antibody
  • Description
    Rabbit polyclonal to Scratch 2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Dog, Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 258-307 (HMQTHSAFKH YRCRQCDKSF ALKSYLHKHC EAACAKAAEP PPPTPAGPAS) of Human Scratch 2 (NP_149120)

  • Positive control
    • Fetal muscle lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • RelevanceScratch 2 belongs to the snail C2H2-type zinc-finger protein family. It contains 5 C2H2-type zinc fingers and may be involved in transcriptional regulation.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Scratch 2 protein antibody
    • Scratch homolog 2, zinc finger protein (Drosophila) antibody
    • SCRT2 antibody

Anti-Scratch 2 antibody images

  • Anti-Scratch 2 antibody (ab83031) at 1 µg/ml (5% skim milk / PBS buffer) + Human fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 33 kDa
    Observed band size : 29 kDa (why is the actual band size different from the predicted?)

References for Anti-Scratch 2 antibody (ab83031)

ab83031 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"