Anti-SCRG1 antibody (ab121384)
Key features and details
- Rabbit polyclonal to SCRG1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SCRG1 antibody -
Description
Rabbit polyclonal to SCRG1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen, corresponding to amino acids 34-98 of Human SCRG1.
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Positive control
- Human cerebral cortex tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Expressed abundantly in the central nervous system of adult, but not at all in fetal brain. High levels of SCRG1 transcripts are also observed in testis and aorta. -
Sequence similarities
Belongs to the SCRG1 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 11341 Human
- Omim: 603163 Human
- SwissProt: O75711 Human
- Unigene: 7122 Human
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Alternative names
- AW124307 antibody
- Scrapie-responsive gene 1 protein antibody
- Scrapie-responsive protein 1 antibody
see all
Images
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Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm. Recommended concentration of ab121384 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab121384 at 1/100 dilution staining SCRG1 in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry, showing strong cytoplasmic positivity in neuronal cells.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121384 has not yet been referenced specifically in any publications.