• Product name
  • Description
    Rabbit polyclonal to SCRIBBLE
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fusion protein corresponding to C terminus of human SCRIBBLE (including PDZ3 and 4).

  • Positive control
    • Diluted human cell extracts.



Our Abpromise guarantee covers the use of ab36708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/5000. Predicted molecular weight: 175 kDa.


  • Function
    Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.
  • Tissue specificity
    Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).
  • Sequence similarities
    Belongs to the LAP (LRR and PDZ) protein family.
    Contains 16 LRR (leucine-rich) repeats.
    Contains 4 PDZ (DHR) domains.
  • Post-translational
    Ubiquitinated; targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded.
  • Cellular localization
    Cell membrane. Cell junction > adherens junction. Cytoplasm. Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. Localizes to neuronal post- and pre-synaptic regions.
  • Information by UniProt
  • Database links
  • Alternative names
    • CRIB 1 antibody
    • CRIB1 antibody
    • hScrib antibody
    • LAP 4 antibody
    • LAP4 antibody
    • PDZ domain protein scribble antibody
    • Protein LAP4 antibody
    • Protein scribble homolog antibody
    • SCRB 1 antibody
    • SCRB1 antibody
    • SCRIB 1 antibody
    • Scrib antibody
    • SCRIB_HUMAN antibody
    • SCRIB1 antibody
    • Scribble antibody
    • Scribble homolog 1 antibody
    • scribbled homolog (Drosophila) antibody
    • Vartul antibody
    see all


  • ICC/IF image of ab36708 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36708, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-SCRIBBLE antibody (ab36708) at 1/5000 dilution

    Lane 1 : Whole cell lysate prepared from prostate cancer cells
    Lane 2 : Whole cell lysate prepared from prostate cancer cells
    Lane 3 : Whole cell lysate prepared from prostate cancer cells

    Lysates/proteins at 40 µg per lane.

    HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 175 kDa
    Observed band size : 175 kDa

    Exposure time : 1 minute

    Image courtesy of an anonymous Abreview.

    No unspecific bands detected.

    See Abreview


ab36708 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (prostate cancer cells)
Loading amount
40 µg
prostate cancer cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Feb 27 2012


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