The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
1/5000. Predicted molecular weight: 175 kDa.
Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.
Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).
Belongs to the LAP (LRR and PDZ) protein family. Contains 16 LRR (leucine-rich) repeats. Contains 4 PDZ (DHR) domains.
Ubiquitinated; targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded.
Cell membrane. Cell junction > adherens junction. Cytoplasm. Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity. Localizes to neuronal post- and pre-synaptic regions.
ICC/IF image of ab36708 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36708, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-SCRIBBLE antibody (ab36708)Image courtesy of an anonymous Abreview.
All lanes : Anti-SCRIBBLE antibody (ab36708) at 1/5000 dilution
All lanes : Whole cell lysate prepared from prostate cancer cells
Lysates/proteins at 40 µg per lane.
Secondary All lanes : HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 175 kDa Observed band size: 175 kDa