Overview

  • Product name
    Anti-SCUBE3 antibody
  • Description
    Rabbit polyclonal to SCUBE3
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human SCUBE3 (internal sequence). The exact sequence is proprietary. 15 amino acid peptide
    Database link: Q8IX30

  • Positive control
    • Human pancreas tissue, Human kidney tissue, Mouse kidney tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab189955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 109 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificity
    Highly expressed in osteoblasts. Expression is low or absent in non-bone tissues.
  • Sequence similarities
    Contains 1 CUB domain.
    Contains 9 EGF-like domains.
  • Post-translational
    modifications
    N-glycosylated.
    May undergo proteolytic cleavage in the C-terminal region.
  • Cellular localization
    Secreted. Cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • CEGF3 antibody
    • CUB and EGF containing protein 3 antibody
    • CUB and EGF-like domain-containing protein 3 antibody
    • CUB domain and EGF like repeat containing 3 antibody
    • DKFZp686B09105 antibody
    • DKFZp686B1223 antibody
    • DKFZp686D20108 antibody
    • FLJ34743 antibody
    • OTTHUMP00000016250 antibody
    • SCUB3_HUMAN antibody
    • SCUBE 3 antibody
    • SCUBE3 antibody
    • Signal peptide antibody
    • Signal peptide CUB and EGF like domain containing protein 3 antibody
    • Signal peptide CUB domain EGF like 3 antibody
    see all

Images

  • Lane 1 : Anti-SCUBE3 antibody (ab189955) at 1 µg/ml
    Lane 2 : Anti-SCUBE3 antibody (ab189955) at 2 µg/ml

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Mouse kidney tissue lysate


    Predicted band size : 109 kDa
  • Immunofluorescence staining of Human kidney tissue, labeling SCUBE3 using ab189955 at 20 ug/ml.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling SCUBE3 using ab189955 at 5 µg/ml

References

ab189955 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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