Overview

  • Product nameAnti-SCUBE3 antibody
    See all SCUBE3 primary antibodies
  • Description
    Mouse monoclonal to SCUBE3
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Mouse, Rat, Chicken, Dog, Human, Pig
  • Immunogen

    GST-tagged recombinant fragment, corresponding to amino acids 29-148 of Human SCUBE3 (AAH52263)

Properties

Applications

Our Abpromise guarantee covers the use of ab76798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight of endogenous protein: 109 kDa.
    Note: this antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Tissue specificityHighly expressed in osteoblasts. Expression is low or absent in non-bone tissues.
    • Sequence similaritiesContains 1 CUB domain.
      Contains 9 EGF-like domains.
    • Post-translational
      modifications
      N-glycosylated.
      May undergo proteolytic cleavage in the C-terminal region.
    • Cellular localizationSecreted. Cell surface.
    • Information by UniProt
    • Database links
    • Alternative names
      • CEGF3 antibody
      • CUB and EGF containing protein 3 antibody
      • CUB and EGF-like domain-containing protein 3 antibody
      • CUB domain and EGF like repeat containing 3 antibody
      • DKFZp686B09105 antibody
      • DKFZp686B1223 antibody
      • DKFZp686D20108 antibody
      • FLJ34743 antibody
      • OTTHUMP00000016250 antibody
      • SCUB3_HUMAN antibody
      • SCUBE 3 antibody
      • SCUBE3 antibody
      • Signal peptide antibody
      • Signal peptide CUB and EGF like domain containing protein 3 antibody
      • Signal peptide CUB domain EGF like 3 antibody
      see all

    Anti-SCUBE3 antibody images

    • Anti-SCUBE3 antibody (ab76798) at 1 µg/ml + immunogen (recombinant GST-tagged fragment from Human protein) at 0.1 µg

      Secondary
      Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

      Predicted band size : 109 kDa
      Observed band size : 39 kDa (why is the actual band size different from the predicted?)

    References for Anti-SCUBE3 antibody (ab76798)

    ab76798 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Mouse Tissue lysate - whole (heart)
    Loading amount 25 µg
    Specification heart
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Dr. Hua Jiang

    Verified customer

    Submitted Aug 21 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"