Overview

  • Product nameAnti-SCXA antibody
    See all SCXA primary antibodies
  • Description
    Rabbit polyclonal to SCXA
  • Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-M, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Horse, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to N-terminal residues of human SCXA

Properties

Applications

Our Abpromise guarantee covers the use of ab58655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000.
WB Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.Can be blocked with SCXA peptide (ab214116). IP enrichment could be done before running a reducing gel. The positive control is purified protein or overpressed cell lysate.
ELISA Use at an assay dependent concentration.
IHC-M Use at an assay dependent concentration. PubMed: 21412429
ICC Use at an assay dependent concentration. PubMed: 21412429

Target

References for Anti-SCXA antibody (ab58655)

This product has been referenced in:
  • Poulsen RC  et al. Cell differentiation versus cell death: extracellular glucose is a key determinant of cell fate following oxidative stress exposure. Cell Death Dis 5:e1074 (2014). WB . Read more (PubMed: 24556689) »
  • Cadby JA  et al. Differences between the cell populations from the peritenon and the tendon core with regard to their potential implication in tendon repair. PLoS One 9:e92474 (2014). WB ; Horse . Read more (PubMed: 24651449) »

See all 12 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Olfactory Stem Cells of Rat (GFP))
Permeabilization Yes - 0,1% Triton X100
Specification Olfactory Stem Cells of Rat (GFP)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Formaldehyde
Username

Dr. Antoine Veron

Verified customer

Submitted Jul 15 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rabbit Cell (Olfactory Stem Cells of Rabbit)
Permeabilization Yes - 0,1% Triton X100
Specification Olfactory Stem Cells of Rabbit
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Formaldehyde
Username

Dr. Antoine Veron

Verified customer

Submitted Jul 15 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Dog Cell (Olfactory stem cells (tenocytes differentiation))
Permeabilization Yes - 0,1 % Triton X100
Specification Olfactory stem cells (tenocytes differentiation)
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Dr. Antoine Veron

Verified customer

Submitted Aug 12 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Horse Cell (Olfactory stem cells)
Permeabilization Yes - 0.1% Triton 100X
Specification Olfactory stem cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Dr. Antoine Veron

Verified customer

Submitted Jun 19 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step PBSMBT (1X PBS, 1.5% Milk, 1.5% BSA, 0.1% Triton X-100) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 4°C
Sample Mouse Tissue sections (E11.5 embryos)
Specification E11.5 embryos
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 06 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Horse Cell (Tenocyte Differentiation)
Specification Tenocyte Differentiation
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Tween 20
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted May 06 2013

Thank you for the blot image. I think the band below the 25 kDa marker is likely to be SCXA. The peptide blocking experiment might confirm this but the peptide might also block binding of the antibody to the other proteins at the higher molecular weigh...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Cow Cell (Tendon)
Specification Tendon
Fixative Formaldehyde
Permeabilization Yes - 0.2% Triton-X- in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Carsten Schnatwinkel

Verified customer

Submitted Jan 11 2013

IHC-M stands for Immunohistochemistry (Methylmethacrylate sections) and was sued in the publication with the following http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=21412429&dopt=Abstract reference. Unfortun...

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I performed an alignment between the immunogen used to generate ab58655 (the immunogen is located in the N terminal region) and the bovine sequence. There is a 94.5% level of homology between the two (there was only 1 amino acid difference).

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"