Overview

  • Product name
  • Description
    Rabbit polyclonal to SCY1 like 3
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 80-559 of Human SCY1 like 3 (NP_065156).

  • Positive control
    • A431 whole cell lysate; HeLa cells; H1299 xenograft; 293T, H1299, HepG2, MOLT4 and Raji cell lines.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: Isoform 1, 83 ; Isoform 2, 77 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function
    May play a role in regulating cell adhesion/migration complexes in migrating cells.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the protein kinase superfamily.
    Contains 4 HEAT repeats.
    Contains 1 protein kinase domain.
  • Domain
    The protein kinase domain is predicted to be catalytically inactive.
  • Post-translational
    modifications
    May be myristoylated; myristoylation may target it to Golgi compartment.
    Phosphorylated.
  • Cellular localization
    Cytoplasm. Golgi apparatus. Cell projection > lamellipodium. Colocalized with EZR/VIL2, actin and CD44 in lamellipodia.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ezrin binding protein PACE 1 antibody
    • Ezrin-binding protein PACE-1 antibody
    • PACE 1 antibody
    • PACE1 antibody
    • PACE1_HUMAN antibody
    • Protein associating with the carboxyl terminal domain of ezrin antibody
    • Protein-associating with the carboxyl-terminal domain of ezrin antibody
    • SCY1 like 3 (S. cerevisiae) antibody
    • SCY1 like 3 antibody
    • SCY1 like protein 3 antibody
    • SCY1 like pseudokinase 3 antibody
    • SCY1-like protein 3 antibody
    • SCYL 3 antibody
    • SCYL3 antibody
    see all

Images

  • Anti-SCY1 like 3 antibody (ab96357) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : Isoform 1, 83 ; Isoform 2, 77 kDa
  • ab96357, at 1/200 dilution, staining SCY1 like 3 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.
  • ab96357, at 1/500 dilution, staining SCY1 like 3 in paraffin-embedded H1299 xenograft by Immunohistochemistry.

References

ab96357 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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