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Rabbit polyclonal to SCY1 like 3
Recombinant fragment, corresponding to a region within amino acids 80-559 of Human SCY1 like 3 (NP_065156).
- A431 whole cell lysate; HeLa cells; H1299 xenograft; 293T, H1299, HepG2, MOLT4 and Raji cell lines.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/3000. Predicted molecular weight: Isoform 1, 83 ; Isoform 2, 77 kDa.
||1/100 - 1/500.
||1/100 - 1/200.
May play a role in regulating cell adhesion/migration complexes in migrating cells.
Belongs to the protein kinase superfamily.
Contains 4 HEAT repeats.
Contains 1 protein kinase domain.
The protein kinase domain is predicted to be catalytically inactive.
May be myristoylated; myristoylation may target it to Golgi compartment.
Cytoplasm. Golgi apparatus. Cell projection > lamellipodium. Colocalized with EZR/VIL2, actin and CD44 in lamellipodia.
Information by UniProt
- Ezrin binding protein PACE 1 antibody
- Ezrin-binding protein PACE-1 antibody
- PACE 1 antibody
Western blot - SCY1 like 3 antibody (ab96357)
Anti-SCY1 like 3 antibody (ab96357) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size : Isoform 1, 83 ; Isoform 2, 77 kDa
Immunocytochemistry/ Immunofluorescence - SCY1 like 3 antibody (ab96357)
ab96357, at 1/200 dilution, staining SCY1 like 3 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SCY1 like 3 antibody (ab96357)
ab96357, at 1/500 dilution, staining SCY1 like 3 in paraffin-embedded H1299 xenograft by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"