The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionRegulates COPI-mediated retrograde traffic. Has no detectable kinase activity in vitro. Isoform 6 acts as transcriptional activator. It binds to three different types of GC-rich DNA binding sites (box-A, -B and -C) in the beta-polymerase promoter region. It also binds to the TERT promoter region.
Sequence similaritiesBelongs to the protein kinase superfamily. Contains 3 HEAT repeats. Contains 1 protein kinase domain.
DomainThe protein kinase domain is predicted to be catalytically inactive.
Cellular localizationNucleus; Cytoplasm > cytoskeleton > centrosome. Endoplasmic reticulum-Golgi intermediate compartment. Golgi apparatus > cis-Golgi network. Localized to the Endoplasmic reticulum-Golgi intermediate and cis-Golgi in an ARF1-independent manner; Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic during interphase and centrosomal during mitosis, it localizes to the centrosomes in a microtubule-independent manner and Cytoplasm. Cytoplasmic throughout the cell cycle.
Anti SCYL1, at 1 µg/ml, staining SCYL1 in HeLa whole cell lysate immunoprecipitated using ab95073 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 3 seconds.
References for Anti-SCYL1 antibody (ab95073)
has not yet been referenced specifically in any publications.
Publishing research using ab95073? Please let us know so that we can cite the reference in this datasheet.
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