Anti-SCYL2 antibody (ab103819)
Key features and details
- Rabbit polyclonal to SCYL2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SCYL2 antibody
See all SCYL2 primary antibodies -
Description
Rabbit polyclonal to SCYL2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human SCYL2 aa 1-950. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Jurkat cell line lysate. SCYL2 transfected 293T cell line lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab103819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 104 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 104 kDa. |
Target
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Function
Component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. According to PubMed:15809293, probable serine/threonine-protein kinase that phosphorylates, in vitro, the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L-lysine. According to PubMed:16914521, has no detectable kinase activity in vitro. May regulate clathrin-dependent trafficking between the TGN and/or the endosomal system. -
Sequence similarities
Belongs to the protein kinase superfamily.
Contains 1 HEAT repeat.
Contains 1 protein kinase domain. -
Post-translational
modificationsAccording to PubMed:15809293, autophosphorylated in presence of poly-L-lysine. -
Cellular localization
Cytoplasm > perinuclear region. Cytoplasmic vesicle > clathrin-coated vesicle. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. According to PubMed:15809293, plasma membrane-associated in clathrin-coated vesicles. According to PubMed:16914521, colocalizes to the trans-Golgi network (TGN) and to endosomal membranes with clathrin, transferrin and plasma membrane adapter AP1 and AP3 complexes. - Information by UniProt
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Database links
- Entrez Gene: 55681 Human
- Omim: 616365 Human
- SwissProt: Q6P3W7 Human
- Unigene: 506481 Human
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Alternative names
- AU015688 antibody
- AW536445 antibody
- BC030932 antibody
see all
Images
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Anti-SCYL2 antibody (ab103819) at 1 µg/ml + Jurkat cell lysate at 50 µg
Predicted band size: 104 kDa -
All lanes : Anti-SCYL2 antibody (ab103819) at 1 µg/ml
Lane 1 : SCYL2 transfected 293T cell line lysate
Lane 2 : Non transfected 293T cell line lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 104 kDa
Datasheets and documents
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Datasheet download
References (1)
ab103819 has been referenced in 1 publication.
- Wojcechowskyj JA et al. Quantitative phosphoproteomics reveals extensive cellular reprogramming during HIV-1 entry. Cell Host Microbe 13:613-23 (2013). WB ; Human . PubMed: 23684312