The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 104 kDa.
Component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. According to PubMed:15809293, probable serine/threonine-protein kinase that phosphorylates, in vitro, the beta2-subunit of the plasma membrane adapter complex AP2 and other proteins in presence of poly-L-lysine. According to PubMed:16914521, has no detectable kinase activity in vitro. May regulate clathrin-dependent trafficking between the TGN and/or the endosomal system.
Belongs to the protein kinase superfamily. Contains 1 HEAT repeat. Contains 1 protein kinase domain.
According to PubMed:15809293, autophosphorylated in presence of poly-L-lysine.
Cytoplasm > perinuclear region. Cytoplasmic vesicle > clathrin-coated vesicle. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. According to PubMed:15809293, plasma membrane-associated in clathrin-coated vesicles. According to PubMed:16914521, colocalizes to the trans-Golgi network (TGN) and to endosomal membranes with clathrin, transferrin and plasma membrane adapter AP1 and AP3 complexes.