The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionPlays a role in the establishment of cell polarity and epithelial lumen formation (By similarity). May play a role in ciliogenesis.
Tissue specificityExpressed in thymus, prostate, testis, ovary, small intestine, colon, mucosa, colon and renal cancer tumors.
Involvement in diseaseSenior-Loken syndrome 7 (SLSN7) [MIM:613615]: A renal-retinal disorder characterized by progressive wasting of the filtering unit of the kidney (nephronophthisis), with or without medullary cystic renal disease, and progressive eye disease. Typically this disorder becomes apparent during the first year of life. Note=The disease is caused by mutations affecting the gene represented in this entry.
Cellular localizationCytoplasm and Cytoplasm > cytoskeleton > centrosome > centriole. Cell junction. Located at the distal ends of both centrioles and colocalizes to centrosomes throughout the cell cycle.