Biotin Anti-SDF1 antibody (ab84277)
Key features and details
- Biotin Rabbit polyclonal to SDF1
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-SDF1 antibody
See all SDF1 primary antibodies -
Description
Biotin Rabbit polyclonal to SDF1 -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
The immunogen is 100% identical across P48061-1 to P48061-6. -
Tested applications
Suitable for: Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human SDF1 aa 22-89. Produced from sera of rabbits pre-immunized with highly pure recombinant Human SDF-1a.
Sequence:KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK
Database link: P48061 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Lyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purification notes
ab84277 was purified by affinity chromatography and then biotinylated.It is sterile filtered. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
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sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit polyclonal to SDF1 (ab9797).
To detect Human SDF-1 alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 - 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ab9797 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF-1 alpha. |
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WB |
Use at an assay dependent concentration.
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Notes |
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Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit polyclonal to SDF1 (ab9797). To detect Human SDF-1 alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 - 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ab9797 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF-1 alpha. |
WB
Use at an assay dependent concentration. |
Target
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Function
Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. -
Tissue specificity
Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Developmental stage
Isoform Alpha is ubiquitously expressed in fetal tissues. Isoform Beta and isoform Delta have more limited expression patterns, with highest levels detected in fetal spleen and fetal liver, respectively. Isoform Gamma and isoform Theta are weakly detected in fetal kidney. -
Post-translational
modificationsProcessed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6387 Human
- Omim: 600835 Human
- SwissProt: P48061 Human
- Unigene: 522891 Human
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Alternative names
- 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 antibody
- AI174028 antibody
- C-X-C motif chemokine 12 antibody
see all
Datasheets and documents
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Datasheet download
References (0)
ab84277 has not yet been referenced specifically in any publications.