The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use at an assay dependent concentration. PubMed: 20871610
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration. PubMed: 25086214
Use at an assay dependent concentration. PubMed: 21224081
1/1000. Predicted molecular weight: 11 kDa.
1/300 - 1/500.
Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.
Belongs to the intercrine alpha (chemokine CxC) family.
Isoform Alpha is ubiquitously expressed in fetal tissues. Isoform Beta and isoform Delta have more limited expression patterns, with highest levels detected in fetal spleen and fetal liver, respectively. Isoform Gamma and isoform Theta are weakly detected in fetal kidney.
Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
Immunohistochemistry (Frozen sections) - Anti-SDF1 antibody (ab25117)Image from Hartman NW et al., PLoS One. 2010 Dec 31;5(12):e15856. Fig 4.; doi:10.1371/journal.pone.0015856; December 31, 2010, PLoS ONE 5(12): e15856.
Immunohistochemical analysis of murine epileptic brain tissue, staining SDF1 alpha with ab25117.
Sections were blocked (2% BSA + 10% normal goat serum in 0.1% Triton X-100) for 1 hour at room temperature before being incubated with primary antibody overnight at 4°C. An AlexaFluor®647-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Western blot - Anti-SDF1 antibody (ab25117)
Recombinant mouse SDF1 alpha
Predicted band size: 11 kDa
Detection of recombinant Mouse SDF1 alpha by Western Blot.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDF1 antibody (ab25117)Image courtesy of an anonymous Abreview.
ab25117 staining SDF1 alpha in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using 10 mM citrate pH 6.0. Samples were then blocked with 10% serum for 30 minutes at 25°C and incubated with ab25117 at a 1/100 dilution for 2 hours at 25°C. The secondary used was an undiluted Alexa Fluor® 488-conjugated goat polyclonal.
Pinheiro D et al. Cytokines, hepatic cell profiling and cell interactions during bone marrow cell therapy for liver fibrosis in cholestatic mice. PLoS One12:e0187970 (2017).
Read more (PubMed: 29176797) »
Du LL & Liu P CXCL12/CXCR4 axis regulates neovascularization and lymphangiogenesis in sutured corneas in mice. Mol Med Rep13:4987-94 (2016).
Read more (PubMed: 27121088) »