Overview

  • Product name
  • Description
    Rabbit polyclonal to SDF1
  • Specificity
    This antibody will react with mouse and rat SDF 1.
  • Tested applications
    Suitable for: IHC-Fr, Flow Cyt, IHC-FoFr, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment corresponding to Mouse SDF1 aa 20-89.
    Database link: P40224

Properties

Applications

Our Abpromise guarantee covers the use of ab25117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 20871610

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-FoFr Use at an assay dependent concentration. PubMed: 25086214
IHC-P Use at an assay dependent concentration. PubMed: 21224081
WB 1/1000. Predicted molecular weight: 11 kDa.
IP 1/300 - 1/500.

Target

  • Function
    Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
  • Tissue specificity
    Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.
  • Sequence similarities
    Belongs to the intercrine alpha (chemokine CxC) family.
  • Developmental stage
    Isoform Alpha is ubiquitously expressed in fetal tissues. Isoform Beta and isoform Delta have more limited expression patterns, with highest levels detected in fetal spleen and fetal liver, respectively. Isoform Gamma and isoform Theta are weakly detected in fetal kidney.
  • Post-translational
    modifications
    Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 antibody
    • AI174028 antibody
    • C-X-C motif chemokine 12 antibody
    • Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) antibody
    • Chemokine (C-X-C motif) ligand 12 antibody
    • Chemokine CXC motif ligand 12 antibody
    • cxcl12 antibody
    • hIRH antibody
    • hSDF-1 antibody
    • Intercrine reduced in hepatomas antibody
    • IRH antibody
    • OTTHUMP00000019491 antibody
    • PBSF antibody
    • Pre-B cell growth-stimulating factor antibody
    • SCYB12 antibody
    • SDF 1 antibody
    • SDF-1 antibody
    • SDF-1-alpha(3-67) antibody
    • SDF-1a antibody
    • SDF-1b antibody
    • SDF1_HUMAN antibody
    • SDF1A antibody
    • SDF1B antibody
    • Stromal cell-derived factor 1 antibody
    • Stromal cell-derived factor 1 delta antibody
    • Stromal cell-derived factor 1 gamma antibody
    • Stromal cell-derived factor 1a antibody
    • Stromal cell-derived factor-1 alpha antibody
    • Thymic lymphoma cell-stimulating factor antibody
    • Tlsf antibody
    • TLSF-a antibody
    • TLSF-b antibody
    • Tlsfa antibody
    • Tlsfb antibody
    • TPAR1 antibody
    see all

Images

  • Immunohistochemical analysis of murine epileptic brain tissue, staining SDF1 alpha with ab25117.

    Sections were blocked (2% BSA + 10% normal goat serum in 0.1% Triton X-100) for 1 hour at room temperature before being incubated with primary antibody overnight at 4°C. An AlexaFluor®647-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
  • + Recombinant mouse SDF1 alpha

    Predicted band size : 11 kDa
    Detection of recombinant Mouse SDF1 alpha by Western Blot.
  • ab25117 staining SDF1 alpha in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using 10 mM citrate pH 6.0. Samples were then blocked with 10% serum for 30 minutes at 25°C and incubated with ab25117 at a 1/100 dilution for 2 hours at 25°C. The secondary used was an undiluted Alexa Fluor® 488-conjugated goat polyclonal.

    See Abreview

References

This product has been referenced in:
  • Du LL & Liu P CXCL12/CXCR4 axis regulates neovascularization and lymphangiogenesis in sutured corneas in mice. Mol Med Rep 13:4987-94 (2016). WB ; Mouse . Read more (PubMed: 27121088) »
  • Lu W  et al. CXCL12/CXCR4 Axis Regulates Aggrecanase Activation and Cartilage Degradation in a Post-Traumatic Osteoarthritis Rat Model. Int J Mol Sci 17:N/A (2016). WB, ICC/IF ; Rat . Read more (PubMed: 27690009) »

See all 25 Publications for this product

Customer reviews and Q&As


The anti-SDF1 alpha antibody (ab25117) has not been tested for its cross reactivity with samples from chicken however as the homology of the chicken protein and that of the mouse protein (against which this antibody has been raised) only share 75%...

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Thank you for your response, your trust and interest in our products..

I would advise you to look through the available antibodies at this site using the search RabMabs button:

http://www.abcam.com/index.html?pageconfig=resource&...

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Thank you for your enquiry and your interest in our products.

I can confirm that ab25117 has been tested and characterized in IHC-Fr, Flow Cyt, IHC-P, WB, IP so it works in immunohistochemistry both on frozen and formalin-fixed paraffin-embed...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.


I am sorry these products did not perform as stated on the datasheet and for the inconveni...

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Thank you for your email.


I am sorry to hear that none of the 3 SDF1 antibodies we have are working for you. Do all 3 antibodies give you the same problem of high background and no band around 10 kDa? How were your samples prepared? And ...

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Thank you for contacting us.

According to Uniprot, Isoform Alphais ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. It also interacts with the N-terminus of CXCR7.

Using this as a basic for cho...

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Thank you for contacting us. I am sorry to hear that the antibody is not performing as previously. Potential tips that could help with obtaining a band are: 1) use more primary antibody 2) check the secondary antibody if it is working ...

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Thank you for your reply. I am sorry for the trouble with this antibody, and your credit note ID is ***. I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways: (1) Re...

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Thank you for your reply and for your patience. I have contacted the lab to ask what percentage gel they used to test the antibody, but I have not received this information yet. However, we would generally recommend using a 20% acrylamide gel fo...

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Thank you for your enquiry. I am sorry to hear that you have been experiencing problems with ab25117 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that...

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