Overview

  • Product nameAnti-SDF1 antibody
    See all SDF1 primary antibodies
  • Description
    Rabbit polyclonal to SDF1
  • Tested applicationsSuitable for: Neutralising, ICC/IF, Indirect ELISA, Sandwich ELISA, WB, ELISA, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human SDF1 aa 22-89.
    Sequence:

    KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK


    Database link: P48061

Properties

Applications

Our Abpromise guarantee covers the use of ab9797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Neutralising Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hSDF1 alpha (100 ng/ml), a concentration of 2-4 µg/ml of this antibody is required.
ICC/IF Use at an assay dependent concentration. PubMed: 21858102
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution). This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF1 alpha.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to SDF1 (Biotin) (ab84277). (using 100 µl/well antibody solution). This antigen affinity purified antibody, in conjunction with ab84277 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF1 alpha.
WB Use at an assay dependent concentration. To detect hSDF-1a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSDF-1a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hSDF-1a by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hSDF-1a.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent dilution.

Target

  • FunctionChemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
  • Tissue specificityIsoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Developmental stageIsoform Alpha is ubiquitously expressed in fetal tissues. Isoform Beta and isoform Delta have more limited expression patterns, with highest levels detected in fetal spleen and fetal liver, respectively. Isoform Gamma and isoform Theta are weakly detected in fetal kidney.
  • Post-translational
    modifications
    Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 antibody
    • AI174028 antibody
    • C-X-C motif chemokine 12 antibody
    • Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) antibody
    • Chemokine (C-X-C motif) ligand 12 antibody
    • Chemokine CXC motif ligand 12 antibody
    • cxcl12 antibody
    • hIRH antibody
    • hSDF-1 antibody
    • Intercrine reduced in hepatomas antibody
    • IRH antibody
    • OTTHUMP00000019491 antibody
    • PBSF antibody
    • Pre-B cell growth-stimulating factor antibody
    • SCYB12 antibody
    • SDF 1 antibody
    • SDF-1 antibody
    • SDF-1-alpha(3-67) antibody
    • SDF-1a antibody
    • SDF-1b antibody
    • SDF1_HUMAN antibody
    • SDF1A antibody
    • SDF1B antibody
    • Stromal cell-derived factor 1 antibody
    • Stromal cell-derived factor 1 delta antibody
    • Stromal cell-derived factor 1 gamma antibody
    • Stromal cell-derived factor 1a antibody
    • Stromal cell-derived factor-1 alpha antibody
    • Thymic lymphoma cell-stimulating factor antibody
    • Tlsf antibody
    • TLSF-a antibody
    • TLSF-b antibody
    • Tlsfa antibody
    • Tlsfb antibody
    • TPAR1 antibody
    see all

Anti-SDF1 antibody images

  • Immunocytochemistry/ Immunofluorescence analysis of Human bone marrow mesenchymal stem cells staining SDF1 alpha (green) using ab9797.

    Cells were either unstimulated (right) or stimulated with direct current (left) before being fixed in formalin and permeabilized in 0.1% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) and an AlexaFluor®488-conjugated anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.
  • ab9797 staining SDF1 alpha in human colorectal adenocarcinoma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.5 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

  • ab9797 staining SDF1 alpha in human kidney tissue by Immunohistochemistry (Frozen sections).
    The tissue was fixed in acetone, blocked with 10% serum for 20 minutes at 21°C and then incubated with ab9797 at 5µg/ml for 1 hour at 21°C. The secondary used was an undiluted HRP conjugated goat polyclonal.

    See Abreview

References for Anti-SDF1 antibody (ab9797)

This product has been referenced in:
  • Shi W  et al. Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury. Acta Biomater 45:247-261 (2016). ICC/IF ; Rat . Read more (PubMed: 27592818) »
  • Tanios F  et al. CXCR4: A Potential Marker for Inflammatory Activity in Abdominal Aortic Aneurysm Wall. Eur J Vasc Endovasc Surg 50:745-53 (2015). WB, IHC ; Human . Read more (PubMed: 26346005) »

See all 10 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Goat Tissue sections (ovary)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Specification ovary
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50µg/mL · Temperature: 25°C
Fixative Paraformaldehyde
Username

Prof. ANA PAULA RIBEIRO RODRIGUES

Verified customer

Submitted Sep 28 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tooth)
Antigen retrieval step None
Permeabilization No
Specification tooth
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 02 2015

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Colon tumor)
Permeabilization Yes - 1x PBS with 0.3% triton-x-100
Specification Colon tumor
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 07 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step 15 min with special blocking serum included in R&D Systems kit as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0 for 10 min
Sample Rat Tissue sections (kidney)
Specification kidney
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 05 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Cell (Supernatants of carcinoma cells)
Specification Supernatants of carcinoma cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Type Indirect
Username

Dr. Kirsten Hattermann

Verified customer

Submitted May 15 2013

DISCOUNT CODE: *
Expiration date: *

I am very pleased to hear you would like to accept our offer and test ab9797 in pig tissue. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Kidney)
Specification Kidney
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Apr 02 2012

Thank you for contacting us and for your interest in our products.

As far as we are aware, ab9797 and ab109854 have only been tested in the species listed on the datasheet. All tested and guaranteedspecies cross-reactivity information is sta...

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Vielen Dank für Ihre Anfrage.

Als CXCL12(SDF1)-Antikörper könnte ich Ihnen die Kaninchen-polyklonalen ab9797 oder ab109854 empfehlen, die wir für IHC an Paraffinschnitten garantieren können:
Click here (or use the following: http://www....

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Thank you for contacting us.

I am happy to let you know that we have the following antibodies available that we can guarantee to work in IHC on paraffin-embedded tissue with human samples:

CXCR4
Rabbit polyclonal ab2074:
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"