Recombinant
RabMAb

Anti-SDF1 antibody [EPR1216] (ab155090)

Overview

  • Product name
    Anti-SDF1 antibody [EPR1216]
    See all SDF1 primary antibodies
  • Description
    Rabbit monoclonal [EPR1216] to SDF1
  • Host species
    Rabbit
  • Specificity
    Mouse cross reactivity based on sequence analysis only. 100% sequence homology for isforms 1-6 (P48061; isoforms 1-6).
  • Tested applications
    Suitable for: Flow Cyt, WB, ICC/IF, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human SDF1 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: P48061

  • Positive control
    • Human SDF1 alpha recombinant protein; Jurkat cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/10000 - 1/50000. Predicted molecular weight: 11 kDa.
ICC/IF 1/100 - 1/250.
IHC-P Use at an assay dependent concentration. PubMed: 27220474
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
    • Tissue specificity
      Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen.
    • Sequence similarities
      Belongs to the intercrine alpha (chemokine CxC) family.
    • Developmental stage
      Isoform Alpha is ubiquitously expressed in fetal tissues. Isoform Beta and isoform Delta have more limited expression patterns, with highest levels detected in fetal spleen and fetal liver, respectively. Isoform Gamma and isoform Theta are weakly detected in fetal kidney.
    • Post-translational
      modifications
      Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 antibody
      • AI174028 antibody
      • C-X-C motif chemokine 12 antibody
      • Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) antibody
      • Chemokine (C-X-C motif) ligand 12 antibody
      • Chemokine CXC motif ligand 12 antibody
      • cxcl12 antibody
      • hIRH antibody
      • hSDF-1 antibody
      • Intercrine reduced in hepatomas antibody
      • IRH antibody
      • OTTHUMP00000019491 antibody
      • PBSF antibody
      • Pre-B cell growth-stimulating factor antibody
      • SCYB12 antibody
      • SDF 1 antibody
      • SDF-1 antibody
      • SDF-1-alpha(3-67) antibody
      • SDF-1a antibody
      • SDF-1b antibody
      • SDF1_HUMAN antibody
      • SDF1A antibody
      • SDF1B antibody
      • Stromal cell-derived factor 1 antibody
      • Stromal cell-derived factor 1 delta antibody
      • Stromal cell-derived factor 1 gamma antibody
      • Stromal cell-derived factor 1a antibody
      • Stromal cell-derived factor-1 alpha antibody
      • Thymic lymphoma cell-stimulating factor antibody
      • Tlsf antibody
      • TLSF-a antibody
      • TLSF-b antibody
      • Tlsfa antibody
      • Tlsfb antibody
      • TPAR1 antibody
      see all

    Images

    • Immunocytochemistry/Immunofluorescence analysis of THP-1 (Human monocytic leukemia cell line) labeling SDF1 with Purified ab155090 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling SDF1 with purified ab155090 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    • Immunofluorescent analysis of Jurkat cells, labeling SDF1 with ab155090 at 1/100 dilution.

    • Anti-SDF1 antibody [EPR1216] (ab155090) at 1/10000 dilution + Human CXCL12 recombinant protein at 0.01 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 11 kDa

    References

    This product has been referenced in:
    • Izumi D  et al. CXCL12/CXCR4 activation by cancer-associated fibroblasts promotes integrin ß1 clustering and invasiveness in gastric cancer. Int J Cancer 138:1207-19 (2016). ICC/IF ; Human . Read more (PubMed: 26414794) »
    • Liu Z  et al. SDF-1/CXCR4 axis induces apoptosis of human degenerative nucleus pulposus cells via the NF-?B pathway. Mol Med Rep 14:783-9 (2016). IHC-P ; Human . Read more (PubMed: 27220474) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Ab89321, Anti-SDF1 antibody [MM0211-9N26]: The blocking effect of this antibody (to CXCL12 induced chemotaxis) was tested using cells over expressing CXCR4. But this data does not exclude the possibility that it may also block CXCL12 induced effect vi...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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