Overview

  • Product name
  • Description
    Goat polyclonal to SDF4
  • Specificity
    ab113413 is expected to recognize both reported isoforms (NP_057631.1; NP_057260.2).
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Goat, Cow, Dog
  • Immunogen

    Synthetic peptide:

    EYKVKFLASKGHSEK

    by a Cysteine residue linker, corresponding to internal sequence amino acids 161-175 of Human SDF4 (NP_057631.1; NP_057260.2).

  • Positive control
    • Jurkat cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab11343 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab113413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).

Target

Images

  • ab113413 (5µg/ml) staining of paraffin embedded Human Kidney following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows preferential staining in the brush borders of epithelial cells.

  • Anti-SDF4 antibody (ab113413) at 0.1 µg/ml + Jurkat cell lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 42 kDa

References

ab113413 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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