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Purified mitochondrial complex II (Cow).
Our Abpromise guarantee covers the use of ab14715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 0.2 µg/ml. Requires heat-induced antigen retrieval where aldehydes are used as fixatives. Use 20min incubation at 90-100°C in 0.1 M Tris/HCl pH 9.5 with 5% urea (wt/vol).|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 0.1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).|
|IHC-P||Use at an assay dependent concentration. PubMed: 20484225|
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