Overview

  • Product nameAnti-SDHB antibody
    See all SDHB primary antibodies
  • Description
    Rabbit polyclonal to SDHB
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide (YRWMIDSRDD FTEERLAKLQ DPFSLYRCHT IMNCTRTCPK GLNPGKAIAE) corresponding to a region within internal sequence amino acids 216-265 of human SDHB (NP_002991).

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab84622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionIron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
  • PathwayCarbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.
  • Involvement in diseaseDefects in SDHB are a cause of susceptibility to pheochromocytoma (PCC) [MIM:171300]. A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent.
    Defects in SDHB are the cause of hereditary paragangliomas type 4 (PGL4) [MIM:115310]; also known as familial non-chromaffin paragangliomas type 4. Paragangliomas refer to rare and mostly benign tumors that arise from any component of the neuroendocrine system. PGL4 is characterized by the development of mostly benign, highly vascular, slow growing tumors in the head and neck. In the head and neck region, the carotid body is the largest of all paraganglia and is also the most common site of the tumors.
    Defects in SDHB are a cause of paraganglioma and gastric stromal sarcoma (PGGSS) [MIM:606864]; also called Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance.
    Defects in SDHB are a cause of Cowden-like syndrome (CWDLS) [MIM:612359]. Cowden-like syndrome is a cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid, kidney and uterus.
  • Sequence similaritiesBelongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.
    Contains 1 2Fe-2S ferredoxin-type domain.
    Contains 1 4Fe-4S ferredoxin-type domain.
  • Cellular localizationMitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CWS2 antibody
    • DHSB_HUMAN antibody
    • FLJ92337 antibody
    • Ip antibody
    • Iron sulfur subunit antibody
    • Iron sulfur subunit of complex II antibody
    • Iron-sulfur subunit of complex II antibody
    • mitochondrial antibody
    • PGL 4 antibody
    • PGL4 antibody
    • SDH 1 antibody
    • SDH antibody
    • SDH1 antibody
    • SDH2 antibody
    • SDH2, homolog of antibody
    • SdhB antibody
    • SDHIP antibody
    • Succinate dehydrogenase [ubiquinone] iron sulfur protein mitochondrial antibody
    • Succinate dehydrogenase [ubiquinone] iron-sulfur subunit antibody
    • succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial antibody
    • Succinate Dehydrogenase 1 Iron Sulfur Subunit antibody
    • Succinate Dehydrogenase 2, S. cerevisiae, homolog of antibody
    • Succinate dehydrogenase complex subunit B iron sulfur antibody
    • Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein antibody
    • succinate dehydrogenase complex, subunit B, iron sulfur (Ip) antibody
    • Succinate dehydrogenase iron sulfur protein antibody
    see all

Anti-SDHB antibody images

  • Anti-SDHB antibody (ab84622) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • Lane 1 : A COXIV antibody used a mitochondria loading control
    Lane 2 : Anti-SDHB antibody (ab84622) at 1/1000 dilution (in 5% milk for 16 hours at 4°C)

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Mouse heart tissue lysate

    Lysates/proteins at 5 µg per lane.

    Secondary
    An HRP-linked Goat anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-SDHB antibody (ab84622)

This product has been referenced in:
  • Renkema GH  et al. SDHA mutations causing a multisystem mitochondrial disease: novel mutations and genetic overlap with hereditary tumors. Eur J Hum Genet N/A:N/A (2014). Human . Read more (PubMed: 24781757) »
  • Qattan AT  et al. Spatial Distribution of Cellular Function: The Partitioning of Proteins between Mitochondria and the Nucleus in MCF7 Breast Cancer Cells. J Proteome Res : (2012). Read more (PubMed: 23051583) »

See all 2 Publications for this product

Product Wall

Abreviews
Application Western blot
Loading amount 150 µg
Gel Running Conditions Reduced Denaturing
Sample Saccharomyces cerevisiae Cell lysate - whole cell (Yeast whole cell)
Specification Yeast whole cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Dec 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"