Overview

  • Product name
    Anti-SDHC antibody [EPR11035(B)]
    See all SDHC primary antibodies
  • Description
    Rabbit monoclonal [EPR11035(B)] to SDHC
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human SDHC (UniProt Q99643).

  • Positive control
    • THP-1, HeLa and fetal liver lysates; Human liver and lung carcinoma tissues; rat liver lysates; THP-1; Mouse brain lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Predicted molecular weight: 19 kDa.

For unpurified use at 1/10000 - 1/50000.

The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

IHC-P 1/700.

For unpurified use at 1/250 -1/500.

  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
    • Pathway
      Carbohydrate metabolism; tricarboxylic acid cycle.
    • Involvement in disease
      Defects in SDHC are the cause of hereditary paragangliomas type 3 (PGL3) [MIM:605373]; also known as autosomal dominant non-chromaffin paragangliomas type 3. Non-chromaffin paragangliomas are usually benign, neural crest derived tumors of parasympathetic ganglia.
      Defects in SDHC are a cause of paraganglioma and gastric stromal sarcoma (PGGSS) [MIM:606864]; also known as Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance.
    • Sequence similarities
      Belongs to the cytochrome b560 family.
    • Cellular localization
      Mitochondrion inner membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CYB560 antibody
      • C560_HUMAN antibody
      • CYBL antibody
      • Cytochrome B large subunit of complex II antibody
      • Integral membrane protein CII-3 antibody
      • mitochondrial antibody
      • PGL3 antibody
      • QPs-1 antibody
      • QPs1 antibody
      • SDH3 antibody
      • sdhC antibody
      • succinate dehydrgenase cytochrome b antibody
      • succinate dehydrogenase 3, integral membrane subunit antibody
      • Succinate dehydrogenase complex subunit C antibody
      • Succinate dehydrogenase complex subunit C integral membrane protein 15kDa antibody
      • Succinate dehydrogenase cytochrome b560 subunit antibody
      • Succinate dehydrogenase cytochrome b560 subunit mitochondrial antibody
      • Succinate dehydrogenase cytochrome b560 subunit, mitochondrial precursor antibody
      • Succinate-ubiquinone oxidoreductase cytochrome B large subunit antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling SDHC with purified  ab155999 at 1:700 dilution (0.2 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • All lanes : Anti-SDHC antibody [EPR11035(B)] (ab155999) at 1/10000 dilution (unpurified)

      Lane 1 : THP-1 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 19 kDa

    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling SDHC with unpurified ab155999 at 1/250 dilution.

    • Anti-SDHC antibody [EPR11035(B)] (ab155999) at 1/50000 dilution (purified) + Rat liver lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 19 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-SDHC antibody [EPR11035(B)] (ab155999) at 1/10000 dilution (purified)

      Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
      Lane 2 : Human fetal liver lysates
      Lane 3 : Mouse brain lysates
      Lane 4 : Rat brain lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 19 kDa
      Observed band size: 19 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling SDHC with unpurified ab155999 at 1/250 dilution.

    References

    This product has been referenced in:
    • Gómez-Serrano M  et al. Differential proteomic and oxidative profiles unveil dysfunctional protein import to adipocyte mitochondria in obesity-associated aging and diabetes. Redox Biol 11:415-428 (2017). WB ; Human . Read more (PubMed: 28064117) »
    • Haller F  et al. Aberrant DNA hypermethylation of SDHC: a novel mechanism of tumor development in Carney triad. Endocr Relat Cancer 21:567-77 (2014). Read more (PubMed: 24859990) »

    See all 3 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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